The measurement of pH is greatly significant in monitoring physiological and biochemical states. In this work, a novel micellar ratiometric fluorescent probe featuring sophisticated energy-transfer (ET) behaviors with p-nitrophenol (PNP) as the energy acceptor and a triplet–triplet annihilation upconversion (TTA-UC) system as the energy donor was designed. The pH-induced molecular configuration of PNP determined the process for the transfer of energy from TTA-UC to PNP. The introduction of the TTA-UC system enabled probe excitation under a long wavelength and afforded a ratiometric signal for pH detection with excellent reliability over diverse interfering factors. This TTA-UC/ET pH probe demonstrated a high sensitivity to hydronium below nanomolar concentrations and an excellent anti-interference ability in serum samples, which provided a novel significant strategy for rapid and accurate detection of blood pH in vitro.

中文翻译：

---

基于三重态敏感上转换和能量转移行为的胶束比例荧光血液 pH 探针

pH的测量对于监测生理和生化状态具有重要意义。在这项工作中，一种新型胶束比率荧光探针具有复杂的能量转移 (ET) 行为，具有p设计了作为能量受体的-硝基苯酚（PNP）和作为能量供体的三重态-三重态湮没上转换（TTA-UC）体系。PNP 的 pH 诱导分子构型决定了能量从 TTA-UC 转移到 PNP 的过程。TTA-UC 系统的引入使探针能够在长波长下激发，并为 pH 检测提供比率信号，在各种干扰因素上具有出色的可靠性。该 TTA-UC/ET pH 探针对低于纳摩尔浓度的水合氢离子具有很高的灵敏度，在血清样品中表现出优异的抗干扰能力，为体外快速准确检测血液 pH 值提供了一种新的重要策略。