The present study deals with the utilization of lignocellulosic hydrolysate-based carbon source for exopolysaccharide (EPS) production using newly reported marine Echinicola sediminis BBL-M-12. This bacterium produced 7.56 g L^–1 and 5.32 g L^–1 of EPS on supplementing 30 g L^–1 glucose and 10 g L^–1 xylose as the sole carbon source, respectively. Whereas on feeding Miscanthus hydrolysate (MCH) with glucose content adjusting to 20 g L^–1, E. sediminis BBL-M-12 produced 6.18 g L^–1 of EPS. The inhibitors study showed bacterium could tolerate higher concentrations of fermentation inhibitors include furfural (0.05%), 5-hydroxymethylfurfural (0.1%), vanillin (0.1%) and acetate (0.5%). Moreover, the EPS composition was greatly altered with the type and concentration of carbon source supplied, although β-D-Glucopyranose, β-D-Galactopyranose, and β-D-Xylopyranose were the dominant monomers detected. Interestingly, E. sediminis BBL-M-12 EPS revealed excellent environmental applications like clay flocculation, oil emulsification, and removal of humic acid, textile dye, and heavy metal from the aqueous phase.

本研究涉及利用新报道的海洋Echinicola sediminis BBL-M-12 使用基于木质纤维素水解物的碳源生产胞外多糖 (EPS)。该细菌在补充 30 g L ^-1葡萄糖和 10 g L ^-1木糖作为唯一碳源时分别产生 7.56 g L ^-1和 5.32 g L ^{-1的 EPS。}饲喂葡萄糖含量调整为 20 g L ^–1的芒草水解物 (MCH) 时，E. sediminis BBL-M-12 产生 6.18 g L ^–1每股收益。抑制剂研究表明，细菌可以耐受更高浓度的发酵抑制剂，包括糠醛（0.05%）、5-羟甲基糠醛（0.1%）、香草醛（0.1%）和醋酸盐（0.5%）。此外，尽管 β-D-吡喃葡萄糖、β-D-吡喃半乳糖和 β-D-吡喃木糖是检测到的主要单体，但 EPS 组成随所提供碳源的类型和浓度而发生很大变化。有趣的是，E. sediminis BBL-M-12 EPS 揭示了出色的环境应用，例如粘土絮凝、油乳化以及从水相中去除腐植酸、纺织染料和重金属。