The anaerobic digestion (AD) of dairy processing wastewater (DPW) to produce bioenergy is considered promising but also associated with the possibility of an unbalanced organic matter and trace metal (TM) content. In this study, the TM content and its impact on AD were determined in an anaerobic membrane bioreactor operated to treat DPW. The results indicated that a deficiency in TMs resulted in the slow deterioration of the process, reducing biogas production, disrupting the buffer system, and the massive accumulation of organic acid. The deficiency of Co/Ni was significant, while iron fluctuated due to microbial and chemical effects. Syntrophic propionate oxidizing bacteria and methanogen were the main groups suppressed under the TM deficient environment, resulting in AD failure. No inhibitory effect on the lactic acid metabolism was observed. Hence, supplying theoretical TM dosage to DPW was necessary to realize the efficient and stable AD process and robust microbial community.

乳制品加工废水 (DPW) 的厌氧消化 (AD) 以生产生物能源被认为是有希望的，但也与有机物和微量金属 (TM) 含量不平衡的可能性有关。在这项研究中，TM 含量及其对 AD 的影响是在用于处理 DPW 的厌氧膜生物反应器中测定的。结果表明，TMs 的缺乏会导致过程缓慢恶化，减少沼气产量，破坏缓冲系统，以及有机酸的大量积累。Co/Ni的缺乏显着，而铁由于微生物和化学作用而波动。在 TM 缺乏的环境下, 共养丙酸氧化菌和产甲烷菌是主要被抑制的群体, 导致 AD 失败。未观察到对乳酸代谢的抑制作用。