With the aim of evaluating the antigerminative activity of plant extracts, a miniaturized assay using 96-well plates (WP assay) was developed and compared to the long-established assay using Petri dishes (PD assay). The WP assay yielded results comparable to those of the PD assay using an ethanolic extract of the Himalayan balsam and lawsone as a standard. It also allowed the needed volume of the test solution to be cut by half and the number of required cress seeds to be cut by more than 1.5. The WP assay was then successfully applied to various extracts of Himalayan balsam, molecules (2,4-dichlorophenoxyacetic acid (2,4-D), glyphosate, and 2-methoxy-1,4-naphthoquinone (2-MNQ)) and target seeds (radish, lettuce, and wheat). By being adapted to a 96-well plate format, the antigerminative WP assay is a promising alternative to the PD assay. Besides, its convenience and low resource consumption make it ready for accelerated and high-throughput screening, as well as automation.

中文翻译：

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用于评估植物提取物和植物毒性化合物抗萌发潜力的快速且可重复的 96 孔板方法

为了评估植物提取物的抗萌发活性，开发了一种使用 96 孔板的小型化测定法（WP 测定法），并与使用培养皿的长期建立的测定法（PD 测定法）进行了比较。WP 测定产生的结果与使用喜马拉雅香脂和指甲花的乙醇提取物作为标准的 PD 测定的结果相当。它还允许将所需的测试溶液体积减少一半，并将所需的水芹种子数量减少 1.5 个以上。WP 测定随后成功应用于喜马拉雅香脂的各种提取物、分子（2,4-二氯苯氧乙酸 (2,4-D)、草甘膦和 2-甲氧基-1,4-萘醌 (2-MNQ)）和靶标种子（萝卜、生菜和小麦）。通过适应 96 孔板格式，抗发芽 WP 测定是 PD 测定的有希望的替代方案。除了，