As a new generation of electrochemiluminescence (ECL) technology, the electrochemical stripping chemiluminescence (ESCL), which combines the advantages of chemiluminescence and stripping voltammetry, has attracted increasing attention in the biosensor field. Here, Ag nanoclusters (NCs) were prepared using DNA double strands as a template and explored to construct an ESCL aptamer sensor (aptasensor) for the detection of a carcinoembryonic antigen (CEA). During the detection process, Ag NCs and the Ag⁺ cations stripped out from Ag NCs were found to respectively catalyze the electrochemical reduction and oxidation of H₂O₂, accelerating the decomposition of H₂O₂ to generate reactive oxygen species (ROSs) and thus effectively enhancing the ECL intensity of luminol. Coupled with the exonuclease I-assisted recycling amplification technology, the developed ESCL aptasensor enabled the sensitive detection of CEA, exhibiting a broad linear response from 100 ag/mL to 10 ng/mL with a low detection limit of 38.86 ag/mL (S/N = 3). In addition, the aptasensor showed good performance in the determination of CEA in human serum with a relative standard deviation (RSD) below 5 %. This work provides an effective method for constructing simple, fast, and sensitive biosensors.

作为新一代电化学发光（ECL）技术，结合化学发光和溶出伏安法优点的电化学溶出化学发光（ESCL）在生物传感器领域越来越受到关注。在这里，以DNA双链为模板制备Ag纳米簇（NCs），并探索构建用于检测癌胚抗原（CEA）的ESCL适体传感器（aptasensor）。在检测过程中，发现Ag NCs和从Ag NCs中剥离出来的Ag ⁺阳离子分别催化H 2 O 2 的电化学还原和氧化_，加速_了H ₂ O _{2的分解。}产生活性氧（ROS），从而有效提高鲁米诺的 ECL 强度。结合核酸外切酶 I 辅助循环扩增技术，开发的 ESCL 适体传感器实现了对 CEA 的灵敏检测，在 100 ag/mL 至 10 ng/mL 范围内表现出宽广的线性响应，检测限低至 38.86 ag/mL (S/ N = 3)。此外，该适体传感器在人血清中CEA的测定中表现出良好的性能，相对标准偏差（RSD）低于5%。这项工作为构建简单、快速、灵敏的生物传感器提供了一种有效的方法。