In the past two decades, a large number of micromixers have been studied and reported to solve the low mass transfer in microchannels. However, there is still a lack of research on how much biochemical reaction efficiency or detection sensitivity can be improved by microfluidic rapid mixing. In this study, using our previously developed ultrafast microelectrokinetic turbulent (μEKT) mixing method, taking glucose oxidation reaction as the research object, we investigated the effect of increasing microfluidic mass transfer efficiency on the efficiency and sensitivity of biochemical reactions. The results showed that fast mixing could improve the enzymatic reaction efficiency by improving the mass transfer efficiency. Further detection of glucose in glucose solutions and blood samples showed that the fast mixing could significantly improve the sensitivity of detection. These results indicate that mixing enhancement can be a significant step of microfluidics-based applications such as POCT, liquid biopsy, food safety, and so on.

中文翻译：

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微流控芯片中电动混合加速生化反应

在过去的二十年中，已经研究并报道了大量的微混合器来解决微通道中的低传质问题。然而，对于微流控快速混合能提高多少生化反应效率或检测灵敏度，目前还缺乏研究。在这项研究中，我们利用我们之前开发的超快微电动湍流（μEKT）混合方法，以葡萄糖氧化反应为研究对象，研究了提高微流体传质效率对生化反应效率和灵敏度的影响。结果表明，快速混合可以通过提高传质效率来提高酶促反应效率。进一步检测葡萄糖溶液和血样中的葡萄糖表明，快速混合可以显着提高检测灵敏度。这些结果表明，混合增强可能是基于微流体的应用（如 POCT、液体活检、食品安全等）的重要一步。