We herein describe a polychromatic quantum dot array (PQDA) to compose a community signal ensemble enabling accurate and precise quantification of miRNAs in a multiplexed manner. Advanced multicomponent ultrahigh-resolution patterning technique achieved by capsulation-assisted transfer printing following self-assembly-based poly(methyl methacrylate) (PMMA) patterning is utilized to manufacture the PQDA, which is designed to discharge a target miRNAs-specific set of fluorescent quantum dots (QDs) through the activity of duplex-specific nuclease (DSN). On the basis of the community signal ensemble produced by the discharged QD profiles, target miRNAs are very specifically identified down to a femtomolar level (1.27 fM) in a multiplexed manner over a wide dynamic range of up to 6 orders of magnitude. The practical diagnostic capability of this strategy is also demonstrated by reliably identifying breast cancer-specific miRNAs from heterogeneous cancer cell lysates.

中文翻译：

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多色量子点阵列构成用于多重 miRNA 检测的群落信号集合

我们在此描述了一个多色量子点阵列 (PQDA) 来组成一个社区信号集合，能够以多路复用的方式准确和精确地量化 miRNA。在基于自组装的聚（甲基丙烯酸甲酯）（PMMA）图案化之后，通过封装辅助转印实现的先进多组分超高分辨率图案化技术被用于制造 PQDA，该 PQDA 旨在释放目标 miRNA 特定的荧光量子组通过双链特异性核酸酶 (DSN) 的活性点 (QD)。基于放电 QD 配置文件产生的社区信号集合，目标 miRNA 在高达 6 个数量级的宽动态范围内以多路复用方式非常具体地识别到飞摩尔水平 (1.27 fM)。