Mungbean (Vigna radiata (L.) R. Wilczek) is an important legume crop of Asia. Salt concentrations typically causes major yield reductions in mungbean. Although the biochemical and genetic basis of salt tolerance-related gene are well studied in Arabidopsis and soybean, limited information concerning the salt tolerance-related genes in mungbean. To address this issue, we mined salt tolerance related genes using the survival rate trait and 160,1405 SNPs in 112 mungbean accessions. As a result, VrFRO8 significantly associated with salt-stress were identified in the GWAS analysis. The candidate gene VrFRO8 was evidenced by comparative genomics, transcriptome and RT-qPCR analysis. The expression level of VrFRO8 was significantly up-regulated (P-value = 0.001) after salt treatment compared with the control group. Moreover, 188 genes and 158 transcription factors related to salt-stress signal transduction pathway were mined, and 18 genes (18/188) had higher expression level in the salt-tolerant varieties than salt-sensitive varieties. And, the function of VrFRO8 was predicted in mungbean, the protein interaction between VrFRO8 and seven related-genes were found by molecular structure analysis. VrFRO8 might reduce SOD contents by influence Fe²⁺/Fe³⁺ ratio under the damage of salt stress. This study used multi-omics data to mine a key genes significantly associated with salt tolerance, and constructed a VrFRO8-related PPI network for salt tolerance, which would lay a solid foundation for further molecular biology research of VrFRO8 and mungbean breeding.

绿豆（Vigna radiata (L.) R. Wilczek）是亚洲重要的豆科作物。盐浓度通常会导致绿豆产量大幅下降。尽管在拟南芥和大豆中对耐盐相关基因的生化和遗传基础进行了很好的研究，但关于绿豆耐盐相关基因的信息有限。为了解决这个问题，我们使用存活率性状和 112 个绿豆种质中的 160,1405 个 SNP 挖掘耐盐相关基因。结果，在 GWAS 分析中确定了与盐胁迫显着相关的VrFRO8 。通过比较基因组学、转录组和 RT-qPCR 分析证明了候选基因VrFRO8 。VrFRO8的表达水平与对照组相比，盐处理后显着上调（P值= 0.001）。此外，还挖掘到了与盐胁迫信号转导通路相关的188个基因和158个转录因子，其中18个基因（18/188）在耐盐品种中的表达水平高于盐敏感品种。并预测了VrFRO8在绿豆中的功能，通过分子结构分析发现了VrFRO8与7个相关基因的蛋白相互作用。VrFRO8可能通过影响盐胁迫下的Fe ²⁺ /Fe ³⁺比值来降低SOD含量。本研究利用多组学数据挖掘了一个与耐盐性显着相关的关键基因，并构建了一个VrFRO8相关的耐盐PPI网络，为进一步开展VrFRO8分子生物学研究和绿豆育种奠定了坚实的基础。