In this study, we performed RNA sequencing of Prx II+/+ and Prx II−/− dermal mesenchymal stem cells (DMSCs) to identify differentially expressed genes (DEGs). To explore the role of Prx II in DMSCs, we performed Gene Ontology analysis of the DEGs. The results showed that the DEGs were mainly involved in the biological processes of cell migration, intercellular adhesion, and coordination of the regulation of stem cell homing. Through the construction of protein–protein interaction network, four hub genes Cd274, Ccl5, Il1b, and Stat1 involved in cell adhesion and cell homing were screened. Quantitative reverse transcription PCR analysis showed that Cd274, Ccl5, Il1b, and Stat1 were down regulated in Prx II−/− DMSCs. miRwalk and Starbase databases were further used to screen the upstream molecules miRNA and lncRNA regulating hub gene. Prx II was found to be involved in the regulation of stem cell homing via the Tctn2/miR-351/Stat1/Il1b axis. Thus, we demonstrated that Prx II is a key molecule in the regulation of the homing ability of DMSCs. Our results provide a theoretical foundation for improving the homing ability of DMSCs by targeting Prx II.

中文翻译：

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使用网络分析鉴定过氧还蛋白 II 及其相关分子作为真皮间充质干细胞归巢的潜在生物标志物

在这项研究中，我们对 Prx II+/+ 和 Prx II-/- 真皮间充质干细胞 (DMSCs) 进行了 RNA 测序，以鉴定差异表达基因 (DEGs)。为了探索 Prx II 在 DMSCs 中的作用，我们对 DEGs 进行了基因本体分析。结果表明，DEGs主要参与细胞迁移、细胞间粘附和协调干细胞归巢调控等生物学过程。通过构建蛋白质-蛋白质相互作用网络，筛选出参与细胞粘附和细胞归巢的4个中枢基因Cd274、Ccl5、Il1b和Stat1。定量逆转录 PCR 分析显示 Cd274、Ccl5、Il1b 和 Stat1 在 Prx II-/- DMSCs 中下调。进一步利用 miRwalk 和 Starbase 数据库筛选上游分子 miRNA 和 lncRNA 调节中枢基因。发现 Prx II 通过 Tctn2/miR-351/Stat1/Il1b 轴参与调节干细胞归巢。因此，我们证明了 Prx II 是调节 DMSCs 归巢能力的关键分子。我们的研究结果为通过靶向 Prx II 提高 DMSCs 的归巢能力提供了理论基础。