Bacterial cellulose (BC) production can be performed using a static or dynamic culture method. In the static culture method, the BC is obtained presents three-dimensional thinner network structures and excellent mechanical properties. In the dynamic culture method, BC is produced in the form of granules or fibrous threads with a lower degree of polymerization, mechanical strength, and crystallinity than those formed in static fermentation. Compared with BC membranes, sphere-like BC (SBC) cultured under dynamic conditions showed advantages for adsorption due to its larger surface area. The objectives of this work were to obtain SBC, by the bacterial strain Komagataeibacter hansenii ATCC 23769, in dynamic culture, using media containing different carbon sources carbon sources, such as fructose (FRU), glucose and sucrose (MS1), sucrose (Y) and glucose (Z and HS), aiming to produce supports for sustained release of rifampicin (RIF). SBC has been produced under agitation at 130 rpm and 25 °C. SBC obtained were processed to remove bacteria and residues from the culture media and lyophilized. The SBC characterizations were performed by Fourier transform infrared spectroscopy, X-ray diffraction, Field emission gun-scanning electron microscopy, and thermogravimetric analysis. The SBC produced were impregnated with antibiotic RIF and tested for the sustained release capacity of this drug by diffusion method and Frans cell kinetics. SBC that the best results for all tests were produced in FRU, Z and MS1 media, respectively. The results demonstrate the potential of the SBC to contribute to the design of new drug delivery systems with biomedical applications.

细菌纤维素 (BC) 生产可以使用静态或动态培养方法进行。在静态培养法中，得到的BC呈现出三维较薄的网状结构和优良的力学性能。在动态培养法中，BC以颗粒或纤维丝的形式产生，其聚合度、机械强度和结晶度低于静态发酵中形成的那些。与 BC 膜相比，在动态条件下培养的球形 BC（SBC）由于其较大的表面积而显示出吸附优势。这项工作的目的是通过菌株Komagataeibacter hansenii获得 SBCATCC 23769，在动态培养中，使用含有不同碳源碳源的培养基，如果糖 (FRU)、葡萄糖和蔗糖 (MS1)、蔗糖 (Y) 和葡萄糖（Z 和 HS），旨在为持续释放利福平（RIF）。SBC 已在 130 rpm 和 25 °C 的搅拌下生产。对获得的 SBC 进行处理以从培养基中去除细菌和残留物并冻干。通过傅里叶变换红外光谱、X 射线衍射、场发射枪扫描电子显微镜和热重分析进行 SBC 表征。生产的 SBC 浸渍抗生素 RIF 并通过扩散法和 Frans 细胞动力学测试该药物的缓释能力。SBC，所有测试的最佳结果分别在 FRU、Z 和 MS1 培养基中产生。