This study aimed to explore the mechanism of actinomycetes inoculation to promote humification based on spectroscopy during straw waste composting. Results showed that inoculating actinomycetes could significantly increase the humification index and humification ratio, which were 2.53% and 21.79% respectively (P < 0.05). A spectroscopic analysis suggested that actinomycetes promoted the relative content of complex components of humic acid and reshaped the structural distribution of two sub fluorescence peaks in it. Furthermore, variance partitioning analysis demonstrated that compared with the intensity, the high-quality uniform distribution of fluorescence peaks had a greater contribution to the improvement of humification. In addition, structural equation model further verified that actinomycetes inoculation promoted the transformation of fulvic acid to humic acid, and then promoted the formation of humic acid. Therefore, actinomycetes inoculation can promote the humification of straw compost by reshaping the complex components of humic acid.

本研究旨在探讨秸秆废弃物堆肥过程中放线菌接种促进腐殖化的机理。结果表明，接种放线菌可显着提高腐殖化指数和腐殖化率，分别为2.53%和21.79%（P < 0.05)。光谱分析表明，放线菌促进了腐植酸复合成分的相对含量，重塑了其中两个亚荧光峰的结构分布。此外，方差分配分析表明，与强度相比，高质量均匀分布的荧光峰对腐殖化的改善贡献更大。此外，结构方程模型进一步验证了放线菌接种促进了黄腐酸向腐植酸的转化，进而促进了腐植酸的形成。因此，放线菌接种可以通过重塑腐植酸的复杂成​​分来促进秸秆堆肥的腐殖化。