A newly designed microbial electrochemical system (MES) with the addition of a baffle between the electrodes was integrated with the anaerobic digestion (AD) process for biogas upgradation. Novel MES configuration attained an increased methane production rate of 292.6 mL/L∙d and methane yield of 0.36 ± 0.006 ${\mathrm{L}}_{{\mathrm{C}\mathrm{H}}_4}$/${\mathrm{g}}_{\mathrm{C}\mathrm{O}\mathrm{D}}$, which were higher than the values (185.3 mL/L∙d and 0.33 ± 0.009 ${\mathrm{L}}_{{\mathrm{C}\mathrm{H}}_4}$/${\mathrm{g}}_{\mathrm{C}\mathrm{O}\mathrm{D}}$) from the MES operation without baffle, respectively. Moreover, the MES with baffle operation resulted in increased substrate removal (88.4 ± 0.5%) and less volatile fatty acids accumulation with a high energy efficiency of 99.6 %. Microbial community analysis revealed that acids metabolizing bacteria, Firmicutes, and Methanothrix were highly enriched in the cathode biofilm of MES with baffle. This study suggests that the baffle addition into the single chamber MES is beneficial to further improve the methanogenesis process for practical applications in the scaled-up MES-AD process.

新设计的微生物电化学系统 (MES) 在电极之间添加了挡板，与厌氧消化 (AD) 工艺相结合，用于沼气升级。新型 MES 配置实现了 292.6 mL/L∙d 的甲烷产率和 0.36 ± 0.006 的甲烷产率${\mathrm{大号}}_{{\mathrm{C}\mathrm{H}}_4}$/${\mathrm{G}}_{\mathrm{C}\mathrm{○}\mathrm{D}}$, 高于值 (185.3 mL/L∙d 和 0.33 ± 0.009${\mathrm{大号}}_{{\mathrm{C}\mathrm{H}}_4}$/${\mathrm{G}}_{\mathrm{C}\mathrm{○}\mathrm{D}}$) 分别来自没有挡板的 MES 操作。此外，带有挡板操作的 MES 可提高底物去除率 (88.4 ± 0.5%) 和更少的挥发性脂肪酸积累，能源效率高达 99.6%。微生物群落分析表明，代谢细菌、厚壁菌门和甲烷丝菌的酸在带挡板的 MES 的阴极生物膜中高度富集。该研究表明，在单室 MES 中添加挡板有利于进一步改进产甲烷过程，以在放大的 MES-AD 过程中实际应用。