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A novel aptamer biosensor based on polydopamine quenched electrochemiluminescence of polyfluorene nanoparticles for amyloid-β oligomers detection
Sensors and Actuators B: Chemical ( IF 8.4 ) Pub Date : 2022-06-14 , DOI: 10.1016/j.snb.2022.132204
Qin Guo, Jun Yang, Jinwen Zhao, Jin Zhang, Ruo Yuan, Shihong Chen

Alzheimer’s disease (AD), as a serious neurodegenerative disease, has posed a great threat to human health. Sensitive detection of its specific biomarker amyloid-β oligomers (AβO) is very important. This work developed a novel electrochemiluminescence (ECL) biosensor for detecting AβO with poly [(9,9-dioctyl-fluorenyl-2,7-diacyl)-alt-co-(9-hexyl-3,6-carbazole)] nanoparticles (PFA NPs) as luminophore and polydopamine (PDA) as ECL quencher. The PFA NPs prepared with poly (styrene-co-maleicanhydride) (PSMA) as carboxyl functionalization reagent were found to have strong ECL emission at + 1.25 V with tripropylamine (TPrA) as coreactant. Furthermore, PFA NPs exhibited charming ECL stability and their ECL signals remained almost unchanged even after a year of storage. The rich carboxyl functional groups on the surface of PFA NPs facilitated the covalent bonding of biomolecules, thus facilitating the construction of “signal-on” ECL aptamer biosensor for detecting AβO. Meanwhile, target switching strategy coupling with three-dimensional (3D) DNA walker cycle amplification endowed aptamer biosensor with excellent analytical performance, characterized by low detection limit (8.30 × 10−15 M) and wide detection range (1.00 × 10−14~1.00 × 10−9 M). This work explored the application of PFA NPs in ECL filed and coupled excellent ECL performance of PFA NPs, efficient ECL quenching effect of PDA, and high sensitivity of target switching strategy for ultrasensitive detection of AβO.



中文翻译:

基于聚多巴胺猝灭的聚芴纳米粒子电化学发光的新型适配体生物传感器用于淀粉样蛋白-β低聚物检测

阿尔茨海默病(Alzheimer's disease,AD)作为一种严重的神经退行性疾病,已对人类健康构成巨大威胁。对其特异性生物标志物淀粉样蛋白-β寡聚体(A β O)的灵敏检测非常重要。这项工作开发了一种用于检测 A β的新型电化学发光 (ECL) 生物传感器O 与聚 [(9,9-二辛基-芴基-2,7-二酰基)-alt-co-(9-己基-3,6-咔唑)] 纳米粒子 (PFA NPs) 作为发光体和聚多巴胺 (PDA) 作为 ECL猝灭剂。发现用聚(苯乙烯-马来酸酐共聚)(PSMA)作为羧基官能化试剂制备的 PFA NPs 在 + 1.25 V 时具有强 ECL 发射,其中三丙胺(TPrA)作为共反应剂。此外,PFA NPs 表现出迷人的 ECL 稳定性,即使经过一年的储存,它们的 ECL 信号也几乎保持不变。PFA NPs表面丰富的羧基官能团促进了生物分子的共价键合,从而有利于构建用于检测Aβ的“signal-on”ECL适配体生物传感器O.同时,目标切换策略与三维(3D)DNA沃克循环扩增相结合,赋予适配体生物传感器优异的分析性能,其特点是检测限低(8.30 × 10 -15 M)和检测范围宽(1.00 × 10 -14 ) ~1.00 × 10 -9 M)。本工作探索了 PFA NPs 在 ECL 领域的应用,并结合了 PFA NPs 优异的 ECL 性能、PDA 的高效 ECL 猝灭效应以及高灵敏度的目标切换策略对 A β O的超灵敏检测。

更新日期:2022-06-16
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