The impact of global warming on biological communities colonizing European alpine ecosystems was recently studied. Hexagonal open top chambers (OTCs) were used for simulating a short-term in situ warming (estimated around 1°C) in some alpine soils to predict the impact of ongoing climate change on resident microbial communities. Total microbial DNA was extracted from soils collected either inside or outside the OTCs over 3 years of study. Bacterial and fungal rRNA copies were quantified by qPCR. Metabarcoding sequencing of taxonomy target genes was performed (Illumina MiSeq) and processed by bioinformatic tools. Alpha- and beta-diversity were used to evaluate the structure of bacterial and fungal communities. qPCR suggests that, although fluctuations have been observed between soils collected either inside and outside the OTCs, the simulated warming induced a significant (p < 0.05) shift only for bacterial abundance. Likewise, significant (p < 0.05) changes in bacterial community structure were detected in soils collected inside the OTCs, with a clear increase of oligotrophic taxa. On the contrary, fungal diversity of soils collected either inside and outside the OTCs did not exhibit significant (p < 0.05) differences, suggesting that the temperature increase in OTCs compared to ambient conditions was not sufficient to change fungal communities.

中文翻译：

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与细菌群落相比，欧洲高山土壤中的真菌群落不受短期原位模拟变暖的影响

最近研究了全球变暖对殖民欧洲高山生态系统的生物群落的影响。六角开顶室 (OTC) 用于模拟短期原位一些高山土壤的变暖（估计约为 1°C），以预测持续的气候变化对居民微生物群落的影响。经过 3 年的研究，从在 OTC 内部或外部收集的土壤中提取了总微生物 DNA。通过 qPCR 定量细菌和真菌 rRNA 拷贝。对分类靶基因进行元条形码测序（Illumina MiSeq）并通过生物信息学工具进行处理。α-和β-多样性被用来评估细菌和真菌群落的结构。qPCR 表明，尽管在 OTC 内部和外部收集的土壤之间观察到波动，但模拟变暖 仅导致细菌丰度的显着 ( p < 0.05) 变化。同样，显着（p < 0.05）在OTCs内收集的土壤中检测到细菌群落结构的变化，贫营养类群明显增加。相反，OTCs 内部和外部收集的土壤的真菌多样性没有表现出显着的 ( p  < 0.05) 差异，这表明与环境条件相比，OTCs 的温度升高不足以改变真菌群落。