The novel Paracoccus denitrificans AC-3 strain was isolated and displayed outstanding purification capability for high concentrations of ammonia nitrogen (NH₄⁺-N) and calcium (Ca²⁺). Meanwhile, the strain exhibited excellent environmental adaptability within a wide pH range and high levels of NH₄⁺-N and Ca²⁺. Nitrogen balance analysis demonstrated that the pathways of NH₄⁺-N removal consisted of 80.12% assimilation and 16.60% heterotrophic nitrification aerobic denitrification (HNAD). In addition, Ca²⁺ was removed by forming calcium carbonate (CaCO₃) with carbonate (CO₃²⁻) and bicarbonate (HCO₃⁻). CO₃²⁻and HCO₃⁻ were obtained from carbon dioxide (CO₂) hydration, which was catalyzed by carbonic anhydrase (CA) secreted by strain AC-3. The alkaline environment for carbonate precipitation was provided by CA and HNAD. The resulting CaCO₃ existed in the form of calcite and exhibited a unique morphology and elemental composition.

分离出新型反硝化副球菌AC-3菌株，对高浓度氨氮（NH ₄ ⁺ -N）和钙（Ca ²⁺）表现出出色的净化能力。同时，该菌株在较宽的pH范围和较高的NH ₄ ⁺ -N和Ca ²⁺含量下表现出优异的环境适应性。氮平衡分析表明NH ₄ ⁺ -N的去除途径包括80.12%的同化和16.60%的异养硝化好氧反硝化(HNAD)。此外，通过与碳酸盐(CO ₃ )形成碳酸钙(CaCO _{3 )来去除Ca} ^2+2- ) 和碳酸氢盐 (HCO ₃ ^- )。CO ₃ ^2-和HCO ₃ ^-是由菌株AC-3 分泌的碳酸酐酶(CA) 催化的二氧化碳(CO _{2 ) 水合获得的。}碳酸盐沉淀的碱性环境由 CA 和 HNAD 提供。生成的CaCO ₃以方解石的形式存在并表现出独特的形态和元素组成。