Glutathione (GSH) is the most abundant biological thiol in cells. It can participate in metabolic processes, regulate the body's oxidation level and is essential for various cell functions. An abnormal concentration of glutathione in the cell is directly related to some diseases. Therefore, it is very important to develop fast, sensitive and reversible detection methods for GSH. Herein, we designed a reversible fluorescent probe (named GeP) for sensing GSH based on the nucleophilic addition and dissociation of intracellular GSH to GeP. The probe GeP showed a fast response time and a 20-fold fluorescence change toward GSH. It exhibited excellent mitochondrial-targeted performance and could be used to monitor GSH in mitochondria. Importantly, GeP could also enable superresolution fluorescence imaging of mitochondria through stochastic optical reconstruction microscopy.

谷胱甘肽 (GSH) 是细胞中最丰富的生物硫醇。它可以参与代谢过程，调节身体的氧化水平，对各种细胞功能至关重要。细胞中谷胱甘肽浓度异常与某些疾病直接相关。因此，开发快速、灵敏、可逆的GSH检测方法非常重要。在此，我们设计了一种可逆荧光探针（命名为GeP），用于基于细胞内 GSH 与GeP的亲核添加和解离来检测 GSH 。探针GeP显示出快速响应时间和对 GSH 的 20 倍荧光变化。它表现出优异的线粒体靶向性能，可用于监测线粒体中的 GSH。重要的是，GeP还可以通过随机光学重建显微镜对线粒体进行超分辨率荧光成像。