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Polyhydroxyalkanoate-driven current generation via acetate by an anaerobic methanotrophic consortium
Water Research ( IF 12.8 ) Pub Date : 2022-06-13 , DOI: 10.1016/j.watres.2022.118743
Xueqin Zhang 1 , Simon J McIlroy 2 , Igor Vassilev 3 , Hesamoddin Rabiee 1 , Manuel Plan 4 , Chen Cai 5 , Bernardino Virdis 1 , Gene W Tyson 2 , Zhiguo Yuan 1 , Shihu Hu 1
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Anaerobic oxidation of methane (AOM) is an important microbial process mitigating methane (CH4) emission from natural sediments. Anaerobic methanotrophic archaea (ANME) have been shown to mediate AOM coupled to the reduction of several compounds, either directly (i.e. nitrate, metal oxides) or in consortia with syntrophic bacterial partners (i.e. sulfate). However, the mechanisms underlying extracellular electron transfer (EET) between ANME and their bacterial partners or external electron acceptors are poorly understood. In this study, we investigated electron and carbon flow for an anaerobic methanotrophic consortium dominated by ‘Candidatus Methanoperedens nitroreducens in a CH4-fed microbial electrolysis cell (MEC). Acetate was identified as a likely intermediate for the methanotrophic consortium, which stimulated the growth of the known electroactive genus Geobacter. Electrochemical characterization, stoichiometric calculations of the system, along with stable isotope-based assays, revealed that acetate was not produced from CH4 directly. In the absence of CH4, current was still generated and the microbial community remained largely unchanged. A substantial portion of the generated current in the absence of CH4 was linked to the oxidation of the intracellular polyhydroxybutyrate (PHB) and the breakdown of extracellular polymeric substances (EPSs). The ability of ‘Ca. M. nitroreducens’ to use stored PHB as a carbon and energy source, and its ability to donate acetate as a diffusible electron carrier expands the known metabolic diversity of this lineage that likely underpins its success in natural systems.



中文翻译:

厌氧甲烷氧化联合体通过乙酸盐驱动的聚羟基烷酸酯发电

甲烷厌氧氧化 (AOM) 是一种重要的微生物过程,可减少天然沉积物中的甲烷 (CH 4 ) 排放。厌氧甲烷氧化古细菌 (ANME) 已被证明可介导 AOM 与几种化合物的还原偶联,这些化合物可以直接(即硝酸盐、金属氧化物)或与同养细菌伙伴(即硫酸盐)联合。然而,对于 ANME 与其细菌伴侣或外部电子受体之间的细胞外电子转移 (EET) 的机制知之甚少。在这项研究中,我们研究了 CH 4中以“Candidatus Methanoperedens nitroreducens 为主的厌氧甲烷氧化菌群的电子和碳流。喂食微生物电解槽(MEC)。醋酸盐被确定为甲烷氧化菌群的可能中间体,它刺激了已知的电活性属Geobacter的生长。电化学表征、系统的化学计量计算以及基于稳定同位素的测定表明乙酸盐不是由 CH 4直接产生的。在没有 CH 4的情况下,电流仍然产生,微生物群落基本保持不变。在没有 CH 4的情况下产生的电流的很大一部分与细胞内聚羟基丁酸酯 (PHB) 的氧化和细胞外聚合物 (EPS) 的分解有关。' Ca的能力。M. nitroreducens 使用储存的 PHB 作为碳和能源,并且它提供醋酸盐作为可扩散电子载体的能力扩展了该谱系的已知代谢多样性,这可能支撑其在自然系统中的成功。

更新日期:2022-06-13
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