The CRISPR-based gene editing technology has become more and more powerful in genome manipulation for agricultural breeding, with numerous improved toolsets springing up. In recent years, many CRISPR toolsets for gene editing, such as base editors (BEs), CRISPR interference (CRISPRi), CRISPR activation (CRISPRa), and plant epigenetic editors (PEEs), have been developed to clarify gene function and full-level gene regulation. Here, we comprehensively summarize the application and capacity of the different CRISPR toolsets in the study of plant gene expression regulation, highlighting their potential application in gene regulatory networks’ analysis. The general problems in CRISPR application and the optimal solutions in the existing schemes for high-throughput gene function analysis are also discussed. The CRISPR toolsets targeting gene manipulation discussed here provide new solutions for further genetic improvement and molecular breeding of crops.

中文翻译：

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使用 CRISPR 工具集剖析植物基因功能以改善作物

基于 CRISPR 的基因编辑技术在农业育种的基因组操作中变得越来越强大，许多改进的工具集如雨后春笋般涌现。近年来，已经开发出许多用于基因编辑的 CRISPR 工具集，例如碱基编辑器 (BEs)、CRISPR 干扰 (CRISPRi)、CRISPR 激活 (CRISPRa) 和植物表观遗传编辑器 (PEE)，以阐明基因功能和全水平。基因调控。在这里，我们全面总结了不同 CRISPR 工具集在植物基因表达调控研究中的应用和能力，突出了它们在基因调控网络分析中的潜在应用。还讨论了CRISPR应用中的普遍问题以及现有高通量基因功能分析方案中的最优解决方案。