Rolling circle amplification (RCA) is a simple and isothermal DNA amplification technique that is used to generate thousands of repeating DNA sequences using circular templates under the catalysis of DNA polymerase. Compared to alternating temperature nucleic acid amplification such as polymerase chain reaction (PCR) amplification, RCA is more suitable for on-spot detection without the need for an expensive thermal cycler. In this study, the principle and classification of RCA are introduced, and the applications of RCA in the detection of pathogenic bacteria, nucleic acid tumor markers, viruses, and proteins are reviewed. Finally, the perspectives of RCA in biological detection are anticipated. The RCA method has a great potential for biological detection. This review aims to provide references for the further development and application of the RCA technique in biosensors.

中文翻译：

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滚环放大生物检测的最新进展：设计策略、生物传感机制和实际应用

滚环扩增 (RCA) 是一种简单的等温 DNA 扩增技术，用于在 DNA 聚合酶的催化下使用环状模板生成数千个重复的 DNA 序列。与聚合酶链式反应 (PCR) 扩增等交变温度核酸扩增相比，RCA 更适合现场检测，无需昂贵的热循环仪。本研究介绍了RCA的原理和分类，综述了RCA在病原菌、核酸肿瘤标志物、病毒和蛋白质检测中的应用。最后，展望了RCA在生物检测中的前景。RCA方法在生物检测方面具有巨大潜力。