Waste-activated sludge (WAS) and greasy sludge (GS) discharged from the canned tuna industry are considerably characterized as harsh organic wastes to be individually treated by using traditional anaerobic digestion. This study was attempted to anaerobically co-digest WAS and GS in continuous pilot scale two-stage process, comprising the first 50 L continuous stir tank reactor (CSTR₁) and the second 250 L continuous stir tank reactor (CSTR₂). The two-stage co-digesting operation of dewatered WAS:GS ratio of 0.4:1 (g-VS) at ambient temperature with the organic loading rate (OLR) of 12.6 ± 0.75 g-VS/L·d and 2.26 ± 0.13 g-VS/L·d, corresponding to 3-day and 17-day hydraulic retention time (HRT) for the first and second stage, respectively generated highest methane production rate of 957 ± 86 mL-CH₄/L·d, corresponding to methane yield of 423.4 ± 36 mL-CH₄/g-VS. Organic removal efficiency obtained was around 67.5% on COD basis. The microbial diversity was depended on the process's activity. Bacteria were mostly detected in the CSTR₁, dominating with the phylum Firmicutes and Proteobacteria, whereas genus Methanosaeta archaea were found dominantly in the CSTR₂. The economic analysis of process shows payback period (PBP), internal rate of return (IRR), and net present value (NPV) of 3 years, 30%, and 250,177 USD, respectively. This study demonstrated the potential approach to applying the two-stage anaerobic co-digestion process to stabilize both WAS and GS along with generating valuable bioenergy carriers.

金枪鱼罐头行业排放的废物活性污泥 (WAS) 和含油污泥 (GS) 具有相当大的特点，它们是需要通过传统厌氧消化进行单独处理的苛刻有机废物。本研究试图在连续中试规模两阶段工艺中厌氧共消化 WAS 和 GS，包括第一个 50 L 连续搅拌釜反应器 (CSTR ₁ ) 和第二个 250 L 连续搅拌釜反应器 (CSTR ₂）。脱水 WAS:GS 比为 0.4:1 (g-VS) 的两级共消化操作在环境温度下有机负载率 (OLR) 分别为 12.6 ± 0.75 g-VS/L·d 和 2.26 ± 0.13 g -VS/L·d，对应于第一阶段和第二阶段的 3 天和 17 天水力停留时间（HRT），分别产生 957 ± 86 mL-CH ₄ /L·d 的最高甲烷产率，对应于甲烷产量为 423.4 ± 36 mL-CH ₄ /g-VS。基于 COD 获得的有机物去除效率约为 67.5%。微生物多样性取决于过程的活动。细菌主要在 CSTR ₁中检测到，以厚壁菌门和变形菌门为主，而在 CSTR _{2中主要发现古生菌属}. 流程的经济分析显示投资回收期 (PBP)、内部收益率 (IRR) 和净现值 (NPV) 分别为 3 年、30% 和 250,177 美元。这项研究展示了应用两阶段厌氧共消化过程来稳定 WAS 和 GS 以及产生有价值的生物能源载体的潜在方法。