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17α-estradiol does not adversely affect sperm parameters or fertility in male mice: implications for reproduction-longevity trade-offs
GeroScience ( IF 5.6 ) Pub Date : 2022-06-11 , DOI: 10.1007/s11357-022-00601-8
José V V Isola 1, 2 , Gabriel B Veiga 3 , Camila R C de Brito 2 , Joao A Alvarado-Rincón 4 , Driele N Garcia 3 , Bianka M Zanini 2 , Jéssica D Hense 3 , Arnaldo D Vieira 2 , Michael Garratt 5 , Bernardo G Gasperin 2 , Augusto Schneider 2, 3 , Michael B Stout 1
Affiliation  

17α-estradiol (17α-E2) is referred to as a nonfeminizing estrogen that was recently found to extend healthspan and lifespan in male, but not female, mice. Despite an abundance of data indicating that 17α-E2 attenuates several hallmarks of aging in male rodents, very little is known with regard to its effects on feminization and fertility. In these studies, we evaluated the effects of 17α-E2 on several markers of male reproductive health in two independent cohorts of mice. In alignment with our previous reports, chronic 17α-E2 treatment prevented gains in body mass, but did not adversely affect testes mass or seminiferous tubule morphology. We subsequently determined that chronic 17α-E2 treatment also did not alter plasma 17β-estradiol or estrone concentrations, while mildly increasing plasma testosterone levels. We also determined that chronic 17α-E2 treatment did not alter plasma follicle-stimulating hormone or luteinizing hormone concentrations, which suggests 17α-E2 treatment does not alter gonadotropin-releasing hormone neuronal function. Sperm quantity, morphology, membrane integrity, and various motility measures were also unaffected by chronic 17α-E2 treatment in our studies. Lastly, two different approaches were used to evaluate male fertility in these studies. We found that chronic 17α-E2 treatment did not diminish the ability of male mice to impregnate female mice, or to generate successfully implanted embryos in the uterus. We conclude that chronic treatment with 17α-E2 at the dose most commonly employed in aging research does not adversely affect reproductive fitness in male mice, which suggests 17α-E2 does not extend lifespan or curtail disease parameters through tradeoff effects with reproduction.



中文翻译:

17α-雌二醇不会对雄性小鼠的精子参数或生育能力产生不利影响:对繁殖与寿命权衡的影响

17α-雌二醇 (17α-E2) 被称为非女性化雌激素,最近发现它可以延长雄性而非雌性小鼠的健康寿命和寿命。尽管大量数据表明 17α-E2 可以减轻雄性啮齿动物的多种衰老特征,但人们对其对女性化和生育能力的影响知之甚少。在这些研究中,我们评估了 17α-E2 对两个独立小鼠组的雄性生殖健康几个标志物的影响。与我们之前的报告一致,长期 17α-E2 治疗可防止体重增加,但不会对睾丸质量或生精小管形态产生不利影响。我们随后确定,长期 17α-E2 治疗也不会改变血浆 17β-雌二醇或雌酮浓度,同时轻度增加血浆睾酮水平。我们还确定,长期 17α-E2 治疗不会改变血浆卵泡刺激素或黄体生成素浓度,这表明 17α-E2 治疗不会改变促性腺激素释放激素神经元功能。在我们的研究中,精子数量、形态、膜完整性和各种活力指标也不受长期 17α-E2 治疗的影响。最后,这些研究使用了两种不同的方法来评估男性生育能力。我们发现,长期 17α-E2 治疗并没有降低雄性小鼠使雌性小鼠受孕的能力,或在子宫中成功植入胚胎的能力。我们得出的结论是,以衰老研究中最常用的剂量进行 17α-E2 的长期治疗不会对雄性小鼠的生殖健康产生不利影响,这表明 17α-E2 不会通过生殖的权衡效应来延长寿命或减少疾病参数。

更新日期:2022-06-12
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