The field of biocatalysis is expanding owing to the increasing demand for efficient low-cost green chemical processes. However, a feasible strategy for achieving product separation, enzyme recovery, and high catalytic efficiency in biocatalysis remains elusive. Herein, we present thermoresponsive Pickering high internal phase emulsions (HIPEs) as controllable scaffolds for efficient biocatalysis; these HIPEs demonstrate a transition between emulsification and demulsification depending on temperature. Ultra-high-surface-area Pickering HIPEs were stabilized by Candida antarctica lipase B immobilized on starch particles modified with butyl glycidyl ether and glycidyl trimethyl ammonium chloride, thus simplifying the separation and reuse processes and significantly improving the catalytic efficiency. In addition, the switching temperature can be precisely tuned by adjusting the degree of substitution of the modified starches to meet the temperature demands of various enzymes. We believe that this system provides a green platform for various interfacial biocatalytic processes of industrial interest.

中文翻译：

---

用于可回收高效界面生物催化的温度响应 Pickering 高内相乳液

由于对高效低成本绿色化学工艺的需求不断增加，生物催化领域正在扩大。然而，在生物催化中实现产物分离、酶回收和高催化效率的可行策略仍然难以捉摸。在这里，我们提出了热响应皮克林高内相乳液（HIPEs）作为高效生物催化的可控支架。这些 HIPE 展示了乳化和破乳之间的转变，具体取决于温度。超高表面积Pickering HIPEs通过固定在丁基缩水甘油醚和缩水甘油基三甲基氯化铵修饰的淀粉颗粒上的南极念珠菌脂肪酶B来稳定，从而简化了分离和再利用过程，显着提高了催化效率。此外，可以通过调节改性淀粉的取代度来精确调节切换温度，以满足各种酶的温度需求。我们相信，该系统为具有工业意义的各种界面生物催化过程提供了一个绿色平台。