Control and prevention of invasive alien species (IAS) are important issues for the environmental management of dam reservoirs. Recently, environmental DNA (eDNA) analysis has been used for monitoring IAS in aquatic ecosystems. In this study, as a simple and user-friendly eDNA method for monitoring IAS, we developed an eDNA chip analysis for monitoring three IAS, largemouth bass Micropterus salmoides, smallmouth bass Micropterus dolomieu and bluegill sunfish Lepomis macrochirus, in dam reservoirs. The eDNA chip analysis using each IAS-specific primer and probe only amplified and detected the target species. The developed eDNA chip detected three IAS from water samples collected in five dam reservoirs, indicating that eDNA chip analysis can be used as a monitoring tool for IAS. However, IAS were not detected by the eDNA chip at some survey sites where the qPCR assays showed low eDNA concentrations, indicating a lower sensitivity of the eDNA chip at low eDNA concentrations. For the establishment of an eDNA chip for biomonitoring, some challenges faced by other techniques like qPCR and metabarcoding remain, such as sensitivity at low target concentrations and “real-time” detections in the field and further improvement of performance in eDNA chip will be required.

外来入侵物种（IAS）的控制和预防是大坝水库环境管理的重要问题。最近，环境 DNA (eDNA) 分析已被用于监测水生生态系统中的 IAS。在这项研究中，作为一种简单且用户友好的用于监测 IAS 的 eDNA 方法，我们开发了一种用于监测三种 IAS 的 eDNA 芯片分析，大嘴鲈鱼Micropterus salmoides，小嘴鲈鱼Micropterus dolomieu和蓝鳃太阳鱼Lepomis macrochirus，在大坝水库中。使用每种 IAS 特异性引物和探针的 eDNA 芯片分析仅扩增和检测目标物种。开发的eDNA芯片从5个大坝水库采集的水样中检测到3个IAS，表明eDNA芯片分析可以作为IAS的监测工具。然而，在 qPCR 测定显示低 eDNA 浓度的一些调查地点，eDNA 芯片未检测到 IAS，这表明 eDNA 芯片在低 eDNA 浓度下的灵敏度较低。为了建立用于生物监测的eDNA芯片，qPCR和metabarcoding等其他技术面临的一些挑战仍然存在，例如低靶浓度的灵敏度和现场“实时”检测，以及eDNA芯片性能的进一步提高。 .