Investigations on nanomedicine involve conventional two dimensional (2D) imaging techniques for observing the nanoparticle internalization at a single time point where various phases of internalization can be overlooked. In contrast, three dimensional (3D) imaging of fluorescent nanoparticles with anticancer potential can be used for obtaining the time course of cellular retention of particles, and cells can be followed for days. This article demonstrates the application of laser scanning confocal microscopy to quantify poly-l-lysine coated fluorescent ZnO nanoparticle retention and reactive oxygen species (ROS) generation using volumetric imaging. Synthesis of these particles allows monitoring of ROS formation, internalization, and cytotoxicity using the same imaging platform that offers an advantage over measurement using various instruments. PLL-coated ZnO particles' ability to induce a significant reduction in cell-viability suggests its potential as a therapeutic agent. The proposed framework opens up a new avenue for investigating mechanistic aspects of ZnO adsorption and the evaluation of therapeutic efficiency.

中文翻译：

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使用激光扫描共聚焦显微镜对 PLL 涂层荧光 ZnO NP 分布和 ROS 积累进行 3D 成像和量化

对纳米医学的研究涉及传统的二维 (2D) 成像技术，用于在单个时间点观察纳米颗粒内化，在该时间点可以忽略内化的各个阶段。相比之下，具有抗癌潜力的荧光纳米颗粒的三维 (3D) 成像可用于获得颗粒细胞保留的时间过程，并且可以跟踪细胞数天。本文演示了激光扫描共聚焦显微镜在量化 poly -l中的应用。-赖氨酸涂层荧光氧化锌纳米颗粒保留和使用体积成像的活性氧 (ROS) 生成。这些颗粒的合成允许使用相同的成像平台监测 ROS 的形成、内化和细胞毒性，这比使用各种仪器进行测量具有优势。PLL 包覆的 ZnO 颗粒诱导细胞活力显着降低的能力表明其作为治疗剂的潜力。所提出的框架为研究 ZnO 吸附的机制方面和治疗效率的评估开辟了一条新途径。