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In silico characterization of Thinopyrum elongatum-derived PsyE1 gene and validation in 7D/7E bread wheat introgression lines open avenues for carotenoid biofortification in wheat
Cereal Research Communications ( IF 1.6 ) Pub Date : 2022-06-09 , DOI: 10.1007/s42976-022-00279-w
Asish Kumar Padhy, Parampreet Kaur, Baljinder Singh, Ravinder Kaur, Sabhyata Bhatia, M. Shamshad, Himanshu Sharma, Satinder Kaur, Puja Srivastava, Achla Sharma

Current global scenario demands agricultural productivity of food grains to be kept at abreast with burgeoning population. Cereals constitute major food stuff for millennia and biofortification of new cereal varieties provides an opportunity to tackle global-scale malnutrition deficiencies without doing major shifts in the diets. Carotenoid biofortification in wheat grains has recently caught the attention of breeders owing to a myriad of health benefits offered by this micronutrient. Thinopyrum elongatum-derived PsyE1 gene encoding for Phytoene Synthase encoding Y gene, is a jackpot to enhance the carotenoid content in wheat. The present study is the first report deciphering detailed in silico characterization of Thinopyrum elongatum-derived PsyE1 gene and its protein. Promoter analysis of chloroplast localized PsyE1 gene provides clues about its possible role in stress resistance along with enhancing the carotenoid content in both durum and bread wheat. Homology, phylogeny and protein modelling studies of PsyE1 revealed its closer evolutionary relationship with barley and wheat, as well as provided a preliminary insight into catalytic and secondary structure of the protein. PCR validation of PsyE1 in 7D/7E bread wheat introgression lines further facilitated development of functional marker that could be used to track its introgression in elite bread wheat varieties. Overall, these detailed insilico insights into structure, function and validation of PsyE1 open doors for its deployment in to produce carotene biofortified hexaploid wheat through facilitating development of functional markers and MAS, as well as to elucidate its mechanism of action and regulation in response to external stimuli.



中文翻译:

长刺槐衍生的 PsyE1 基因的计算机表征和在 7D/7E 面包小麦渗入系中的验证为小麦中的类胡萝卜素生物强化开辟了途径

当前的全球形势要求粮食的农业生产力与不断增长的人口保持同步。谷物是数千年来的主要食物,新谷物品种的生物强化提供了一个机会,可以在不改变饮食结构的情况下解决全球范围内的营养不良问题。小麦籽粒中的类胡萝卜素生物强化最近引起了育种者的注意,因为这种微量营养素提供了无数的健康益处。长穗刺麦草衍生的 PsyE1基因编码八氢番茄红素合成酶编码 Y 基因,是提高小麦中类胡萝卜素含量的大奖。本研究是第一份详细解读长刺参的计算机表征的报告PsyE1基因及其蛋白。叶绿体定位的PsyE1基因的启动子分析提供了有关其在抗逆性中的可能作用以及提高硬粒小麦和面包小麦中类胡萝卜素含量的线索。PsyE1的同源性、系统发育和蛋白质建模研究揭示了它与大麦和小麦更密切的进化关系,并提供了对该蛋白质催化和二级结构的初步了解。PsyE1在 7D/7E 面包小麦基因渗入系中的 PCR 验证进一步促进了功能标记的开发,该标记可用于跟踪其在优质面包小麦品种中的基因渗入。总体而言,这些对insilico结构、功能和验证的详细见解PsyE1通过促进功能标记和 MAS 的开发,以及阐明其响应外部刺激的作用和调节机制,为其部署用于生产胡萝卜素生物强化六倍体小麦打开了大门。

更新日期:2022-06-10
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