Characterization and genetic engineering of plant transporters involved in the pesticide uptake and translocation facilitate pesticide relocation to the tissue where the pests feed, thus improving the bioavailability of the agrichemicals. We aimed to identify thiamethoxam (THX) transporters in rice and modify their expression for better brown planthopper (BPH) control with less pesticide application. A yeast library expressing 1385 rice transporters was screened, leading to the identification of an amino acid transporter-like (ATL) gene, namely OsATL15, which facilitates THX uptake in both yeast cells and rice seedlings. In contrast to a decrease in THX content in osatl15 knockout mutants, ectopic expression of OsATL15 under the control of the CaMV 35S promoter or a vascular-bundle-specific promoter gdcsPpro significantly increased THX accumulation in rice plants, thus further enhancing the THX efficacy against BPH. OsATL15 was localized in rice cell membrane and abundant in the root transverse sections, vascular bundles of leaf blade, and stem longitudinal sections, but not in hull and brown rice at filling stages. Our study shows that OsATL15 plays an essential role in THX uptake and its systemic distribution in rice. OsATL15 could be valuable in achieving precise pest control by biotechnology approaches.

中文翻译：

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氨基酸转运蛋白样蛋白 (OsATL15) 促进噻虫嗪在水稻中的系统分布，以控制褐飞虱

参与农药吸收和转运的植物转运蛋白的表征和基因工程有助于农药重新定位到害虫进食的组织，从而提高农用化学品的生物利用度。我们的目的是鉴定水稻中的噻虫嗪 (THX) 转运蛋白并修改其表达，以便在减少农药施用的情况下更好地控制褐飞虱 (BPH)。筛选了表达 1385 个水稻转运蛋白的酵母文库，鉴定出类氨基酸转运蛋白 (ATL) 基因，即OsATL15，该基因促进酵母细胞和水稻幼苗吸收 THX。与osatl15敲除突变体中THX含量的减少相反， CaMV 35S启动子或维管束特异性启动子gdcsPpro控制下的OsATL15异位表达显着增加了水稻植株中THX的积累，从而进一步增强了THX对抗BPH的功效。OsATL15定位于水稻细胞膜，在根横切面、叶片维管束和茎纵切面丰富，但在灌浆期的稻壳和糙米中不存在。我们的研究表明OsATL15在 THX 吸收及其在水稻中的全身分布中起着重要作用。OsATL15对于通过生物技术方法实现精确害虫控制具有重要价值。