Withania somnifera (L.) has long been used as a traditional rasayana herb against a variety of human ailments. This research presents a high performance thin layer chromatography based chemo profiling of Ashvagandharishta and its antidepressant activity. The in-house formulation was made using a fermentation process according to the Indian Pharmacopoeia. Physiochemical standardization of the formulation was performed using different quality control parameters such as total ash, acid insoluble ash, alcohol soluble extract value and water soluble extract value. A column chromatography and high performance thin layer chromatography method was used to isolate and estimation of withanolide-A, withaferin-A & β-sitosterol from the root of W. somnifera. In addition. The antidepressant effect of different formulations were carried out by force swimming test in albino mice. The thiobarbituric acid reactive substances (TBARS) and Glutathione (GSH) assay was used to find out the oxidative stress. W. somnifera root has been standardized macroscopically, microscopically, physico-chemically according to the guidelines of the Ayurvedic Pharmacopoeia. The qualitative and quantitative analysis was performed using high performance thin layer chromatography and it was performed on each formulation and found the content of withanolide-A and -sitosterol in the in-house formulation is higher while withaferin-A is rather contained in the decoction. The antidepressant effect showed that the immobility time was lowest in the case of the standard formulation followed by in house formulation, while the increase in glutathione and the reduction in thiobarbituric acid reactive substances levels revealed the antioxidant nature of the formulation. In conclusion, based on the above results, we can conclude that Ashvagandharishta could be a breakthrough for the treatment of depression in the future.

Withania somnifera (L.) 长期以来一直被用作对抗各种人类疾病的传统 rasayana 草药。这项研究提出了一种基于高性能薄层色谱的 Ashvagandharishta 化学分析及其抗抑郁活性。内部配方是根据印度药典使用发酵工艺制成的。使用不同的质量控制参数，如总灰分、酸不溶性灰分、醇溶性提取物值和水溶性提取物值，对制剂进行理化标准化。采用柱层析和高效薄层层析法从催眠西药的根中分离和鉴定了withanolide-A、withaferin-A和β-谷甾醇。. 此外。在白化病小鼠中通过力量游泳试验进行不同制剂的抗抑郁作用。硫代巴比妥酸反应物质（TBARS）和谷胱甘肽（GSH）测定用于找出氧化应激。睡梦茄根据阿育吠陀药典的指导方针，根已在宏观、微观、物理化学上进行了标准化。使用高效薄层色谱法对每个制剂进行定性和定量分析，发现内部制剂中的withanolide-A和-谷甾醇含量较高，而汤剂中含有较多的withaferin-A。抗抑郁作用表明，标准制剂的静止时间最低，其次是内部制剂，而谷胱甘肽的增加和硫代巴比妥酸反应物质水平的降低揭示了该制剂的抗氧化性质。总之，基于上述结果，