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Study on the morpho-anatomy of Lepisorus species through light microscopy and scanning electron microscopy and its systematic implications
Microscopy Research and Technique ( IF 2.5 ) Pub Date : 2022-06-09 , DOI: 10.1002/jemt.24174
Sinjini Mondal 1 , Saurav Moktan 1
Affiliation  

The genus Lepisorus is often associated with complex lineages in Polypodiaceae, which leads to difficulty in understanding taxonomic relationships among the species. The taxa is mostly epiphytic with some distinct features like rhizomes covered with clathrate scales, simple fronds, sori in a single row, intermixed with peltate paraphyses. In this study, we precisely focus on the Lepisorus species and seek a broader understanding of the taxonomic relationships that prevail in this genus. The morpho-anatomical traits of rhizomes, stipe, lamina, and sporangia were explored in nine species with the aid of light microscopy and scanning electron microscopy techniques for gathering the qualitative and quantitative data sets. These character traits were resolved into groups based on degree of similarity and principal component analysis to comprehend the covariance among the variables. The data were then employed to prepare an artificial dichotomous key and hierarchical cluster analysis was established that revealed five related clades with eight members whereas one member emerged as outgroup. The results correspond with other recent phylogenetic studies of the concerned genus hence confirming immense reliability and thrust of light microscopy, scanning electron microscopy, and morphology-based studies that are being less utilized in fern taxonomy.

中文翻译:

光学显微镜和扫描电镜研究鳞翅目鱼的形态解剖及其系统意义

Lepisorus属通常与Polypodiaceae 中的复杂谱系有关,这导致难以理解物种之间的分类关系。该类群大多是附生的,具有一些明显的特征,如覆盖有笼形鳞片的根茎、简单的叶状体、单排的sori,与盾状的旁骨混合。在这项研究中,我们精确地关注Lepisorus物种并寻求对该属中普遍存在的分类关系的更广泛理解。借助光学显微镜和扫描电子显微镜技术收集定性和定量数据集,研究了 9 个物种的根茎、菌柄、叶片和孢子囊的形态解剖特征。根据相似度和主成分分析将这些性格特征分组,以了解变量之间的协方差。然后将数据用于准备人工二分键并建立分层聚类分析,该分析揭示了五个相关的进化枝,其中有八个成员,而一个成员作为外群出现。
更新日期:2022-06-09
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