Abstract Nanoparticles synthesized from plants are being explored for cancer treatment therapies all over the world. This study reported the eco-friendly and low-cost method for the green synthesis of silver nanoparticles (AgNPs) from Adansonia digitata fruit as a reducing and capping agent. The anti-cancer potential of synthesized particles was explored against HTC116 and SW480 colon cancer cell lines. Prepared AgNPs were characterized by ultraviolet-visible spectroscopy, zeta potential, transmission electronic microscopy, scanning electronic microscopy, Fourier transform infrared, and energy-dispersive spectrum. The cytotoxicity was determined with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and expression levels of four genes (CTNNB1, APC, LRP5, and LRP6) were checked by reverse transcription polymerase chain reaction. The sharp peak of surface plasmon resonance at 400 nm confirms the formation of AgNPs. Dynamic light scattering showed average sizes of 16.34 nm with a polydispersity index of 0.193. A. digitata AgNPs were spherical with slight aggregated. AgNPs were more cytotoxic than A. digitata extract and decrease the expression of CTNNB1 and LRP6 genes while LRP5 gene expression was increased in both cell lines. APC gene expression was decreased in SW480 but increased in HTC116 with treatment. Overall, this study suggested that AgNPs synthesized by A. digitata fruit extract can be an attractive candidate for anticancer applications.

中文翻译：

---

Adansonia digitata 果实提取物绿色合成银纳米粒子对 HTC116 和 SW480 人结肠癌细胞系的细胞毒性

摘要 世界各地都在探索从植物中合成的纳米粒子用于癌症治疗。本研究报道了一种绿色合成银纳米粒子 (AgNPs) 的环保且低成本的方法，该方法来自 Adansonia digitata 果实作为还原剂和封端剂。针对 HTC116 和 SW480 结肠癌细胞系探索了合成颗粒的抗癌潜力。通过紫外-可见光谱、zeta 电位、透射电子显微镜、扫描电子显微镜、傅里叶变换红外和能量色散光谱对制备的 AgNPs 进行了表征。用 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 法和四个基因 (CTNNB1, APC, LRP5, 和 LRP6) 通过逆转录聚合酶链反应进行检查。表面等离子体共振在 400 nm 处的尖峰证实了 AgNPs 的形成。动态光散射显示平均尺寸为 16.34 nm，多分散指数为 0.193。A. digitata AgNPs 呈球形，有轻微聚集。AgNPs 比 A. digitata 提取物更具细胞毒性，并降低 CTNNB1 和 LRP6 基因的表达，而 LRP5 基因表达在两种细胞系中均增加。APC 基因表达在 SW480 中降低，但在 HTC116 中随着治疗的增加而增加。总体而言，这项研究表明，由 A. digitata 果实提取物合成的 AgNPs 可以成为抗癌应用的有吸引力的候选者。34 nm，多分散指数为 0.193。A. digitata AgNPs 呈球形，有轻微聚集。AgNPs 比 A. digitata 提取物更具细胞毒性，并降低 CTNNB1 和 LRP6 基因的表达，而 LRP5 基因表达在两种细胞系中均增加。APC 基因表达在 SW480 中降低，但在 HTC116 中随着治疗的增加而增加。总体而言，这项研究表明，由 A. digitata 果实提取物合成的 AgNPs 可以成为抗癌应用的有吸引力的候选者。34 nm，多分散指数为 0.193。A. digitata AgNPs 呈球形，有轻微聚集。AgNPs 比 A. digitata 提取物更具细胞毒性，并降低 CTNNB1 和 LRP6 基因的表达，而 LRP5 基因表达在两种细胞系中均增加。APC 基因表达在 SW480 中降低，但在 HTC116 中随着治疗的增加而增加。总体而言，这项研究表明，由 A. digitata 果实提取物合成的 AgNPs 可以成为抗癌应用的有吸引力的候选者。