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Elasmobranch diversity across a remote coral reef atoll revealed through environmental DNA metabarcoding
Zoological Journal of the Linnean Society ( IF 2.8 ) Pub Date : 2022-04-14 , DOI: 10.1093/zoolinnean/zlac014
Nicholas Dunn 1, 2, 3 , Vincent Savolainen 1 , Sam Weber 4 , Samantha Andrzejaczek 5 , Chris Carbone 3 , David Curnick 3
Affiliation  

Abstract As elasmobranchs are becoming increasingly threatened, efficient methods for monitoring the distribution and diversity of elasmobranch populations are required. Environmental DNA (eDNA) metabarcoding is a progressively applied technique that enables mass identification of entire communities and is an effective method for the detection of rare and elusive species. We performed an eDNA metabarcoding survey for fish communities around a coral reef atoll in the Chagos Archipelago (Central Indian Ocean) and assessed the diversity and distribution of elasmobranch species detected within these communities. Our eDNA survey detected 353 amplicon sequence variants (ASVs) attributed to fishes, 12 of which were elasmobranchs. There were no differences in fish communities based on the presence and absence of ASVs between sample depth (surface and 40 m) or sampling habitat, but communities based on read abundance were significantly different between habitats. The dominant elasmobranch species were grey reef (Carcharhinus amblyrhynchos) and silvertip (C. albimarginatus) sharks, and elasmobranch communities were significantly different between sampling depth and habitat. Overall, we find that eDNA metabarcoding can be used to reveal the diversity of elasmobranchs within broader taxonomic assays, but further research and development of targeted metabarcoding primers may be required before it can be integrated into a toolkit for monitoring these species.

中文翻译:

通过环境 DNA 元条形码揭示了一个偏远珊瑚礁环礁的 Elasmobranch 多样性

摘要随着 elasmobranchs 的威胁越来越大,需要有效的方法来监测 elasmobranch 种群的分布和多样性。环境 DNA (eDNA) 元条形码是一种逐渐应用的技术,可以大规模识别整个群落,是检测稀有和难以捉摸物种的有效方法。我们对查戈斯群岛(中印度洋)珊瑚礁环礁周围的鱼类群落进行了 eDNA 元条形码调查,并评估了在这些群落中检测到的 elasmobranch 物种的多样性和分布。我们的 eDNA 调查检测到 353 个扩增子序列变体 (ASV) 归因于鱼类,其中 12 个是 elasmobranchs。根据样本深度(表面和 40 m)或采样栖息地之间是否存在 ASV,鱼类群落没有差异,但基于读取丰度的群落在栖息地之间存在显着差异。主要的弹性鳃类物种是灰礁鲨(Carcharhinus amblyrhynchos)和银鳍鲨(C. albimarginatus),并且弹性鳃类群落在采样深度和栖息地之间存在显着差异。总体而言,我们发现 eDNA 元条形码可用于在更广泛的分类分析中揭示弹性分支的多样性,但可能需要进一步研究和开发靶向元条形码引物,然后才能将其整合到监测这些物种的工具包中。主要的弹性鳃类物种是灰礁鲨(Carcharhinus amblyrhynchos)和银鳍鲨(C. albimarginatus),并且弹性鳃类群落在采样深度和栖息地之间存在显着差异。总体而言,我们发现 eDNA 元条形码可用于在更广泛的分类分析中揭示弹性分支的多样性,但可能需要进一步研究和开发靶向元条形码引物,然后才能将其整合到监测这些物种的工具包中。主要的弹性鳃类物种是灰礁鲨(Carcharhinus amblyrhynchos)和银鳍鲨(C. albimarginatus),并且弹性鳃类群落在采样深度和栖息地之间存在显着差异。总体而言,我们发现 eDNA 元条形码可用于在更广泛的分类分析中揭示弹性分支的多样性,但可能需要进一步研究和开发靶向元条形码引物,然后才能将其整合到监测这些物种的工具包中。
更新日期:2022-04-14
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