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Cytogenetic characterisation and chromosomal mapping of microsatellite and telomeric repeats in two gecko species (Reptilia, Gekkonidae) from Thailand
Comparative Cytogenetics ( IF 1 ) Pub Date : 2021-02-02 , DOI: 10.3897/compcytogen.v15.i1.58208
Weera Thongnetr , Surachest Aiumsumang , Rodjarin Kongkaew , Alonglod Tanomtong , Chatmongkon Suwannapoom , Sumalee Phimphan

Studies of chromosomes of Cyrtodactylus jarujini Ulber, 1993 and C. doisuthep Kunya et al., 2014 to compare microsatellite and TTAGGG sequences by classical and molecular techniques were conducted in Thailand. Karyological typing from a conventional staining technique of C. jarujini and C. doisuthep showed diploid chromosome numbers of 40 and 34 while the Fundamental Numbers (NF) were 56 in both species. In addition, we created the chromosome formula of the chromosomes of C. jarujini showing that 2n (40) = Lsm1 + Lsm2 + Lt3 + Mm1 + Mt4 + Sm2 + Sa2 + St5 while that of C. doisuthep was 2n (34) = Lsm3 + Lm2 + Lt3 + Mm1 + Mt2 + Sm4 + Sa1 + St1. Ag-NOR staining revealed NOR-bearing chromosomes in chromosome pairs 13 and 14 in C. jarujini, and in chromosome pairs 9 and 13 in C. doisuthep. This molecular study used the FISH technique, as well as microsatellite probes including (A)20, (TA)15, (CGG)10, (CGG)10, (GAA)10, (TA)15 and TTAGGG repeats. The signals showed that the different patterns in each chromosome of the Gekkonids depended on probe types. TTAGGG repeats showed high distribution on centromere and telomere regions, while (A)20, (TA)15, (CGG)10, (CGG)10, (GAA)10 and (TA)15 bearing dispersed over the whole genomes including chromosomes and some had strong signals on only a pair of homologous chromosomes. These results suggest that the genetic linkages have been highly differentiated between the two species.

中文翻译:

泰国两种壁虎(爬行动物、壁虎科)微卫星和端粒重复序列的细胞遗传学特征和染色体作图

在泰国进行了 Cyrtodactylus jarujini Ulber 染色体的研究,1993 年和 C. doisuthep Kunya 等人,2014 年,以通过经典和分子技术比较微卫星和 TTAGGG 序列。来自 C. jarujini 和 C. doisuthep 的常规染色技术的核型分型显示,这两个物种的二倍体染色体数分别为 40 和 34,而基本数字 (NF) 为 56。此外,我们创建了 C. jarujini 染色体的染色体公式,显示 2n (40) = Lsm1 + Lsm2 + Lt3 + Mm1 + Mt4 + Sm2 + Sa2 + St5 而 C. doisuthep 的染色体公式是 2n (34) = Lsm3 + Lm2 + Lt3 + Mm1 + Mt2 + Sm4 + Sa1 + St1。Ag-NOR 染色显示 C. jarujini 的第 13 和 14 号染色体对以及 C. doisuthep 的第 9 和 13 对染色体中含有 NOR 的染色体。这项分子研究使用了 FISH 技术,以及微卫星探针,包括 (A)20、(TA)15、(CGG)10、(CGG)10、(GAA)10、(TA)15 和 TTAGGG 重复序列。信号表明,Gekkonids 的每条染色体中的不同模式取决于探针类型。TTAGGG 重复在着丝粒和端粒区域显示出高度分布,而 (A)20、(TA)15、(CGG)10、(CGG)10、(GAA)10 和 (TA)15 分散在整个基因组中,包括染色体和有些仅在一对同源染色体上具有强信号。这些结果表明,这两个物种之间的遗传联系已经高度分化。TTAGGG 重复在着丝粒和端粒区域显示出高度分布,而 (A)20、(TA)15、(CGG)10、(CGG)10、(GAA)10 和 (TA)15 分散在整个基因组中,包括染色体和有些仅在一对同源染色体上具有强信号。这些结果表明,这两个物种之间的遗传联系已经高度分化。TTAGGG 重复在着丝粒和端粒区域显示出高度分布,而 (A)20、(TA)15、(CGG)10、(CGG)10、(GAA)10 和 (TA)15 分散在整个基因组中,包括染色体和有些仅在一对同源染色体上具有强信号。这些结果表明,这两个物种之间的遗传联系已经高度分化。
更新日期:2021-02-02
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