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Karyotype and COI sequences of Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera,Chironomidae) from the bay of Orkhon River, Mongolia
Comparative Cytogenetics ( IF 1 ) Pub Date : 2021-05-27 , DOI: 10.3897/compcytogen.v15.i2.66549
Viktor V. Bolshakov , Alexander A. Prokin

Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera, Chironomidae) is recorded from Mongolia for the first time. Eleven banding sequences determined in the Mongolian population were previously known from Altai and Yenisei populations: sokA1, sokB1, sokB2, sokC1, sokC2, sokD1, sokD2, sokE1, sokF1, sokF2 and sokG1. The additional B-chromosomes are absent. DNA-barcoding of COI gene was carried out for this species for the first time. The phylogenetic tree estimated by Bayesian inference showed a high similarity of the studied species with Ch. acutiventris Wülker, Ryser et Scholl, 1983 from the Chironomus obtusidens-group. The estimated genetic distance K2P between Ch. sokolovae and Ch. acutiventris is much lower (0.38%) than the commonly accepted threshold of 3% for species of genus Chironomus Meigen, 1803. Our results show that the accepted cytogenetic criteria of species level in the genus Chironomus are more in accordance with morphological ones of the same level, than with molecular-genetic criteria accepted for species COI genetic distance.

中文翻译:

蒙古鄂尔浑河湾 Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera, Chironomidae) 的核型和 COI 序列

Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera, Chironomidae) 首次从蒙古记录。在蒙古人群中确定的 11 个条带序列以前从阿尔泰和叶尼塞人群中得知:sokA1、sokB1、sokB2、sokC1、sokC2、sokD1、sokD2、sokE1、sokF1、sokF2 和 sokG1。没有额外的 B 染色体。首次对该物种进行COI基因的DNA条形码编码。通过贝叶斯推断估计的系统发育树显示所研究物种与 Ch 的高度相似性。acutiventris Wülker,Ryser et Scholl,1983 年来自 Chironomus obtusidens 组。Ch之间的估计遗传距离K2P。sokolovae 和 Ch。acutiventris 远低于(0.38%)摇蚊 Meigen 属物种普遍接受的阈值 3%,1803。
更新日期:2021-05-27
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