In this study, steel slag powder and fly ash were used as reinforcement and modification materials, and their effects on the growth, survival, and nucleation of mineral admixtures on bacteria were analyzed. Electrical conductivity, urease activity, and zeta potential tests were conducted, and the results showed that: (1) different mineral environments had different effects on the urease activity of Sporosarcina pasteurii. The steel slag powder solution inhibited urease activity, while the fly ash solution promoted urease activity. (2) S. pasteurii showed different growth characteristics in different mineral environments, especially in the fly ash solution, which showed higher hydrolysis efficiency, urease activity, and calcium carbonate yield. (3) The zeta potential test results showed that the nucleation of mineral admixtures on bacteria started to occur when the calcium ion concentration in the mineral filtrate was higher than 15 mmol/L. Different calcium ion concentrations showed greater impacts on the nucleation rate of mineral admixtures. The results of this study indicated the adaptability of microorganisms in the steel slag powder and fly ash solutions and the nucleation characteristics of calcium carbonate on the microbial surface, providing a theoretical and experimental basis for the subsequent development of new sand-fixing materials.

中文翻译：

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矿物掺合料中的微生物生长和碳酸钙成核特性

本研究以钢渣粉和粉煤灰为增强改性材料，分析了它们对矿物掺合料对细菌的生长、存活和成核的影响。进行了电导率、脲酶活性和zeta电位测试，结果表明：（1）不同的矿物环境对巴氏孢子星脲酶活性的影响不同。钢渣粉溶液抑制脲酶活性，而粉煤灰溶液促进脲酶活性。(2)巴氏杆菌在不同的矿物环境中表现出不同的生长特性，尤其是在粉煤灰溶液中，表现出更高的水解效率、脲酶活性和碳酸钙产率。(3)zeta电位测试结果表明，矿物滤液中钙离子浓度高于15 mmol/L时，矿物外加剂开始在细菌上成核。不同的钙离子浓度对矿物外加剂的成核率影响更大。本研究结果表明了微生物在钢渣粉和粉煤灰溶液中的适应性以及微生物表面碳酸钙的成核特性，为后续开发新型固沙材料提供了理论和实验依据。