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Distribution of interstitial cells of Cajal in the Esophagus and change in distribution after thoracic trauma
Journal of Molecular Histology ( IF 3.2 ) Pub Date : 2022-06-04 , DOI: 10.1007/s10735-022-10074-w
Zhen-Peng Huang 1 , Ke Wang 2, 3 , Hu Qiu 4 , Jia-Wei He 5 , Ling-Wang Kong 5 , Meng Bai 3 , Yi-Xin Cheng 3
Affiliation  

Interstitial cells of Cajal (ICCs) function as pacemaker cells in the gastrointestinal tract. Acute thoracic trauma is a common and lethal cause of death due to physical trauma caused by traffic accidents. This study aimed to explore the distribution of esophageal ICCs and distribution changes observed after acute thoracic trauma. Thirty rabbits were randomly divided into a control group and two study groups. The control group animals underwent an esophagectomy. All animals in the study groups underwent right chest puncture using the Hopkinson bar technique. The study groups were subjected to esophagectomy 24 and 72 h after chest puncture. Distribution, morphology, and density of esophageal ICCs were detected using transmission electron microscopy, toluidine blue staining, and immunohistochemistry. Apoptosis of esophageal ICCs was evaluated using the terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling assay. Western blotting and reverse transcription polymerase chain reaction were used to detect changes in the SCF/c-kit signaling pathway. Esophageal ICCs distribution and SCF/c-kit signal pathway decreased from the upper part to the lower part in both physiological state and after thoracic trauma. In contrast, death of ICCs increased from the upper part to the lower part, both in physiological and injured state (P < 0.05). After thoracic trauma, increased ICCs and decreased death of ICCs in all parts of the esophagus (P < 0.05) were observed. The observed distribution and changes in esophageal ICCs would have an impact on motility and motility disorders of the esophagus.



中文翻译:

Cajal间质细胞在食管内的分布及胸部外伤后分布的变化

Cajal 间质细胞 (ICC) 在胃肠道中起起搏细胞的作用。急性胸外伤是交通事故造成的身体创伤导致的常见致死原因。本研究旨在探讨急性胸外伤后食管ICCs的分布及分布变化。三十只兔子被随机分为对照组和两个研究组。对照组动物接受食管切除术。研究组中的所有动物都使用霍普金森棒技术进行了右胸穿刺。研究组在胸腔穿刺后 24 小时和 72 小时接受食管切除术。使用透射电子显微镜、甲苯胺蓝染色和免疫组织化学检测食管 ICC 的分布、形态和密度。使用末端脱氧核苷酸转移酶 (TdT) dUTP 缺口末端标记测定法评估食管 ICC 的细胞凋亡。Western印迹和逆转录聚合酶链反应用于检测SCF/c-kit信号通路的变化。食管ICCs分布和SCF/c-kit信号通路在生理状态和胸外伤后均由上至下减少。相比之下,生理状态和损伤状态下ICCs的死亡从上到下逐渐增加(P < 0.05)。胸部外伤后食管各部位ICCs增多,ICCs死亡减少(P < 0.05)。观察到的食管 ICC 分布和变化会对食管的运动和运动障碍产生影响。

更新日期:2022-06-06
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