A direct mass spectrometry method utilizing reactive paper spray ionization was developed for sensitive cannabinoid quantitation in biofluid matrices. The ca. 2-minute sample measurements used on-paper derivatization to significantly increase paper spray mass spectrometry (PS-MS) positive ion mode sensitivity while minimizing sample preparation steps. Calibrations demonstrate high linearity, with R² > 0.99 for (−)-trans-Δ⁹-tetrahydrocannabinol (THC) in oral fluid and 11-nor-9-carboxy-Δ⁹-tetrahydrocannabinol (THC-COOH) in urine. The limit of detection and lower limit of quantitation were 0.78 and 10 ng mL⁻¹ for THC in oral fluid and 1.3 and 10 ng mL⁻¹ for THC-COOH in urine, respectively. THC-COOH levels measured by reactive PS-MS in seven spiked human urine samples showed bias of −9.4 to 5.9%, and percent difference values of −16.8 to 9.8% in comparison with a reference LC-MS method. Based upon the method simplicity, validation experiments, sensitivity, and rapidity, we conclude that reactive PS-MS has potential applications for rapid cannabinoid drug testing in urine and oral fluid.

中文翻译：

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利用反应纸喷雾电离直接质谱法定量尿液和口腔液中的大麻素

开发了一种利用反应纸喷雾电离的直接质谱方法，用于生物流体基质中的敏感大麻素定量。约。2 分钟样品测量使用纸上衍生化显着提高纸喷雾质谱 (PS-MS) 正离子模式灵敏度，同时最大限度地减少样品制备步骤。校准显示出高线性度，口腔液中的 (-)-反式-Δ ⁹ - 四氢大麻酚 (THC) 和尿液中的 11-nor-9-羧基-Δ ⁹ - 四氢大麻酚 (THC-COOH) 的R ² > 0.99 。口腔液中 THC的检测限和定量下限分别为 0.78 和 10 ng mL ^-1以及 1.3 和 10 ng mL ^-1分别用于尿液中的 THC-COOH。与参考 LC-MS 方法相比，通过反应性 PS-MS 测量的七份人尿样品中的 THC-COOH 水平显示偏差为 -9.4 至 5.9%，百分比差异值为 -16.8 至 9.8%。基于方法的简单性、验证实验、灵敏度和快速性，我们得出结论，反应性 PS-MS 在尿液和口腔液中快速大麻素药物检测具有潜在应用。