The Spanish Mediterranean basin is particularly susceptible to climate change and human activities, making it vulnerable to the influence of anthropogenic contaminants. Therefore, conducting comprehensive and exhaustive water quality assessment in relevant water bodies of this basin is pivotal. In this work, surface water samples from coastal lagoons or estuaries were collected across the Spanish Mediterranean coastline and subjected to target and suspect screening of 1,585 organic micropollutants by liquid chromatography coupled to ion mobility separation and high resolution mass spectrometry. In total, 91 organic micropollutants could be confirmed and 5 were tentatively identified, with pharmaceuticals and pesticides being the most prevalent groups of chemicals. Chemical analysis data was compared with data on bioanalysis of those samples (recurrent aryl hydrocarbon receptor (AhR) activation, and estrogenic receptor (ER) inhibition in wetland samples affected by wastewater streams). The number of identified organic contaminants containing aromatic rings could explain the AhR activation observed. For the ER antagonistic effects, predictions on estrogenic inhibition potency for the detected compounds were used to explain the activities observed. The integration of chemical analysis with bioanalytical observations allowed a comprehensive overview of the quality of the water bodies under study.

西班牙地中海盆地特别容易受到气候变化和人类活动的影响，因此容易受到人为污染物的影响。因此，对流域相关水体进行全面、详尽的水质评价至关重要。在这项工作中，在西班牙地中海沿岸收集了来自沿海泻湖或河口的地表水样本，并通过液相色谱结合离子淌度分离和高分辨率质谱对 1,585 种有机微污染物进行目标和可疑筛选。总共可以确认 91 种有机微污染物，初步确定 5 种，其中药物和农药是最普遍的化学物质。将化学分析数据与这些样品的生物分析数据（受废水流影响的湿地样品中的复发性芳烃受体 (AhR) 激活和雌激素受体 (ER) 抑制）进行了比较。已鉴定的含有芳环的有机污染物的数量可以解释观察到的 AhR 活化。对于 ER 拮抗作用，使用对检测到的化合物的雌激素抑制效力的预测来解释观察到的活性。将化学分析与生物分析观察相结合，可以全面了解所研究水体的质量。已鉴定的含有芳环的有机污染物的数量可以解释观察到的 AhR 活化。对于 ER 拮抗作用，使用对检测到的化合物的雌激素抑制效力的预测来解释观察到的活性。将化学分析与生物分析观察相结合，可以全面了解所研究水体的质量。已鉴定的含有芳环的有机污染物的数量可以解释观察到的 AhR 活化。对于 ER 拮抗作用，使用对检测到的化合物的雌激素抑制效力的预测来解释观察到的活性。将化学分析与生物分析观察相结合，可以全面了解所研究水体的质量。