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Regulation of tomato fruit elongation by transcription factor BZR1.7 through promotion of SUN gene expression
Horticulture Research ( IF 8.7 ) Pub Date : 2022-05-31 , DOI: 10.1093/hr/uhac121
Ting Yu 1 , Guo Ai 1 , Qingmin Xie 1 , Wenqian Wang 1 , Jianwen Song 1 , Jiaying Wang 1 , Jingbao Tao 1 , Xingyu Zhang 1 , Zonglie Hong 2 , Yongen Lu 1 , Jie Ye 1 , Yuyang Zhang 1 , Junhong Zhang 1 , Zhibiao Ye 1
Affiliation  

Fruit shape is an important biological trait that is also of special commercial value in tomato. The SUN gene has been known as a key regulator of tomato fruit elongation for years, but the molecular mechanisms underlying its transcriptional regulation remain little understood. Here, a unique BZR1-like transcription factor, BZR1.7, was identified as a trans-acting factor of the SUN gene promoter that bound to the conserved E-box of the promoter to promoted SUN gene expression. Overexpression of BZR1.7 in tomato led to elevated SUN gene expression and formation of elongated fruits. Plants of the BZR1.7 knock out mutant created by gene editing did not exhibit observable fruit shape phenotype, suggesting possible functional redundancy of BZR1-like genes in tomato. There were seven BZR1-like genes in the tomato genome and overexpression of BZR1.5 and BZR1.6 displayed similar elongated fruit phenotypes as observed in the BZR1.7 overexpression lines, further supporting the notion of functional redundancy of BZR1-like genes in tomato fruit shape specification. Microscopic analysis revealed that there was decreased number of cell layers in the fruit pericarp in the BZR1.7 overexpression lines. These findings offer new insights into the regulatory mechanism by which BZR1.7 promotes SUN gene expression and regulates fruit elongation in tomato.

中文翻译:

转录因子 BZR1.7 通过促进 SUN 基因表达调控番茄果实伸长

果形是番茄重要的生物学性状,也具有特殊的商业价值。多年来,SUN 基因一直被认为是番茄果实伸长的关键调节因子,但其转录调控的分子机制仍知之甚少。在这里,一种独特的 BZR1 样转录因子 BZR1.7 被鉴定为 SUN 基因启动子的反式作用因子,它与启动子的保守 E-box 结合以促进 SUN 基因表达。BZR1.7 在番茄中的过表达导致 SUN 基因表达升高和细长果实的形成。通过基因编辑产生的 BZR1.7 敲除突变体的植物没有表现出可观察到的果实形状表型,这表明番茄中 BZR1 样基因可能存在功能冗余。番茄基因组中有7个BZR1样基因和BZR1的过表达。5 和 BZR1.6 显示出与 BZR1.7 过表达品系中观察到的相似的细长果实表型,进一步支持了 BZR1 样基因在番茄果实形状规范中的功能冗余概念。显微镜分析表明,在 BZR1.7 过表达株系中,果皮细胞层数减少。这些发现为 BZR1.7 促进 SUN 基因表达和调节番茄果实伸长的调节机制提供了新的见解。
更新日期:2022-05-31
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