Fusarium culmorum (Fc) is a hemibiotrophic plant fungal pathogen that causes crown and root rot disease in wheat. In this study, for the first time, a comparative proteomic analysis was made in seedling roots of two wheat cultivars Falat (susceptible) and Sumai3 (resistant) to understand defense responses induced during the early stage of Fc-infection. The patterns of differentially expressed protein spots (DEPs) were located on two-dimensional gel electrophoresis 48 h after F. culmorum inoculation and a total of 30 major protein spots were successfully identified by mass spectrometry. These proteins were grouped into diverse functional categories which were involved in cellular metabolism (70%), defense (10%), energy (10%) and cell structure (3%), while only two proteins were unknown (7%). Seven DEPs were equally up-regulated with significant expression changes in both cultivars, while seventeen DEPs such as isocitrate dehydrogenase, malate dehydrogenase, enolase which are related to cellular respiration besides lipoxygenase that is vital enzyme in jasmonate biosynthesis were up-regulated only in Sumai3. Likewise, RuBisCO, as a photosynthesis responsive DEPs, showed significantly higher abundance only in Falat. These results showed that the resistant cultivar provides required energy for combating pathogen by reducing photosynthesis and increasing cellular respiration during early stage of Fc-infection. Moreover, lipoxygenase as main marker of octadecanoid pathways solitary increased in resistant cv. Sumai3 and there is a direct correlation between expressed proteins and level of defense responses to crown and root rot disease in wheat.

Fusarium culmorum (Fc) 是一种半营养性植物真菌病原体，可引起小麦的冠腐病和根腐病。在这项研究中，首次对两个小麦品种 Falat（易感）和 Sumai3（抗性）的幼苗根系进行了比较蛋白质组学分析，以了解在 Fc感染早期诱导的防御反应。F. culmorum后 48 小时，差异表达蛋白斑点 (DEP) 的模式位于二维凝胶电泳上接种成功，质谱共鉴定出30个主要蛋白点。这些蛋白质分为不同的功能类别，涉及细胞代谢（70%）、防御（10%）、能量（10%）和细胞结构（3%），而只有两种蛋白质未知（7%）。7 个 DEP 在两个品种中同样上调，表达发生显着变化，而与细胞呼吸有关的异柠檬酸脱氢酶、苹果酸脱氢酶、烯醇化酶等 17 个 DEP 除了在茉莉酸生物合成中至关重要的酶脂氧合酶外，仅在 Sumai3 中上调。同样，RuBisCO 作为一种光合作用响应型 DEP，仅在法拉特中显示出显着更高的丰度。这些结果表明，抗病品种通过在 Fc 感染早期减少光合作用和增加细胞呼吸，为对抗病原体提供了所需的能量。此外，脂氧合酶作为十八烷类途径的主要标志物，在抗性品种中单独增加。Sumai3 表达的蛋白质与小麦对冠腐病和根腐病的防御反应水平之间存在直接相关性。