A microfluidic platform for the integration of multi-step biological assays has been developed. The presented system is a unique instrument compatible with microfluidic chips for various applications based on bead manipulation. Two examples of microfluidic cartridges are presented here. The first one contains two rows of eight chambers (40 and 80 μL), six reagent inlets, eight testing solution (calibrators and samples) inlets and eight outlets to reproduce precisely each step of a biological assay. This configuration is versatile enough to integrate many different biological assays and save a lot of development time. The second architecture is dedicated to one specific protocol and is completely automated from the standard and sample dilutions to the optical detection. Linear dilutions have been integrated to prepare automatically a range of standard concentrations and outlets have been modified for integrated colorimetric detection. The technology uses pneumatically collapsible chambers to perform all the fluidic operations for a fully automated protocol such as volume calibrations, fluid transport, mixing, and washing steps. A programmable instrument with a software interface has been developed to adapt rapidly a protocol to this cartridge. As an example, these new microfluidic cartridges have been used to successfully perform an immunoassay for gluten detection in the dynamic range of 10–30 ppm with good sensitivity (2 ppm) and specificity.

中文翻译：

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用于基于磁珠的定量协议的多功能自动化微流控平台：应用于麸质检测

已经开发了用于集成多步生物测定的微流体平台。所提出的系统是一种独特的仪器，与微流控芯片兼容，适用于基于珠子操作的各种应用。此处介绍了微流控墨盒的两个示例。第一个包含两排八个室（40 和 80 μL）、六个试剂入口、八个测试溶液（校准物和样品）入口和八个出口，以精确再现生物测定的每个步骤。这种配置足够通用，可以集成许多不同的生物测定并节省大量开发时间。第二种架构专用于一个特定的协议，从标准和样品稀释到光学检测都是完全自动化的。已集成线性稀释以自动制备一系列标准浓度，并且已修改出口以集成比色检测。该技术使用气动可折叠室来执行全自动协议的所有流体操作，例如体积校准、流体传输、混合和清洗步骤。一种带有软件界面的可编程仪器已被开发出来，以快速适应该试剂盒的协议。例如，这些新型微流控盒已成功用于在 10–30 ppm 的动态范围内以良好的灵敏度 (2 ppm) 和特异性进行面筋检测的免疫测定。该技术使用气动可折叠室来执行全自动协议的所有流体操作，例如体积校准、流体传输、混合和清洗步骤。一种带有软件界面的可编程仪器已被开发出来，以快速适应该试剂盒的协议。例如，这些新型微流控盒已成功用于在 10–30 ppm 的动态范围内以良好的灵敏度 (2 ppm) 和特异性进行面筋检测的免疫测定。该技术使用气动可折叠室来执行全自动协议的所有流体操作，例如体积校准、流体传输、混合和清洗步骤。一种带有软件界面的可编程仪器已被开发出来，以快速适应该试剂盒的协议。例如，这些新型微流控盒已成功用于在 10–30 ppm 的动态范围内以良好的灵敏度 (2 ppm) 和特异性进行面筋检测的免疫测定。