This paper is the first report of a non-competitive fluorescence polarization immunoassay (NC-FPIA) using a peptide as a tracer. The NC-FPIA can easily and quickly quantify the target after simply mixing them together. This feature is desirable for point-of-need applications such as clinical diagnostics, infectious disease screening, on-site analysis for food safety, etc. In this study, the NC-FPIA was applied to detect CD9, which is one of the exosome markers. We succeeded in detecting not only CD9 but also CD9 expressing exosomes derived from HeLa cells. This method can be applied to various targets if a tracer for the target can be prepared, and expectations are high for its future uses.

中文翻译：

---

使用肽作为示踪剂的非竞争性荧光偏振免疫传感用于 CD9 检测

本文是第一份使用肽作为示踪剂的非竞争性荧光偏振免疫测定法 (NC-FPIA) 的报告。只需将目标混合在一起，NC-FPIA 就可以轻松快速地量化目标。此功能适用于临床诊断、传染病筛查、食品安全现场分析等即时应用。在本研究中，NC-FPIA 用于检测 CD9，它是外泌体之一标记。我们不仅成功地检测到了 CD9，而且还成功检测了源自 HeLa 细胞的表达 CD9 的外泌体。如果可以为目标准备跟踪器，这种方法可以应用于各种目标，并且对其未来用途的期望很高。