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Fabricate, advancement, molecular docking and DNA reactivity of preferred divalent metal(II) complexes attributing (E)-N’-((6-hydroxybenzo[d]oxazol-5-yl) methylene)isonicotinohydrazide
Nucleosides, Nucleotides & Nucleic Acids ( IF 1.3 ) Pub Date : 2022-05-30 , DOI: 10.1080/15257770.2022.2081704
Patil Pratiba 1 , Virupaxappa S Betageri 1 , M S Latha 2 , B Vinay Kumar 3 , B S Ravindranath 4 , C C Vidyasagar 5
Affiliation  

Abstract

A series of metal(II) complexes (M=Co (II), Ni(II) and Cu(II)) supported by Schiff base ligand (L=(E)-N’-((6-hydroxybenzo[d]oxazol-5-yl)methylene)isonicotinohydrazide) has been designed and developed from condensation of 6-hydroxybenzo[d]oxazole-5-carbaldehyde and isoniazid. The ligand (H2L) and its metal(II) complexes were structurally characterized utilizing a variety of physicochemical and spectroscopic approaches. The study shows that Schiff bases (H2L) act as monobasic tridentate ONO ligand and conform to octahedral geometry according to the general formula [M(HL)2]. Furthermore, the interaction of these complexes with CT-DNA was investigated at pH = 7.2, utilizing UV–visible absorption, and viscosity measurement. In order to determine the mechanism of binding of the metal(II) complexes to the B-DNA dodecamer, docking studies were conducted using an AutoDock Vina 1.2.0 tool. The photo induced cleavage reveals that the ligand (H2L) and its complexes have UV–visible photo nuclease properties against pUC19 DNA by agarose gel electrophoresis technique. Studies showed that the complexes evaluated firmly bind to CT-DNA via intercalative mode and provides a distinctive pattern of DNA binding.



中文翻译:

归因于 (E)-N'-((6-羟基苯并[d]恶唑-5-基)亚甲基)异烟酰肼的优选二价金属 (II) 配合物的制造、改进、分子对接和 DNA 反应性

摘要

由席夫碱配体( L =( E ) -N '-((6-羟基苯并[ d ]恶唑))负载的一系列金属(II)配合物( M =Co(II)、Ni(II)和Cu(II)) -5-基)亚甲基)异烟肼)是由 6-羟基苯并[ d ]恶唑-5-甲醛和异烟肼缩合设计和开发的。利用各种物理化学和光谱方法对配体 (H 2 L) 及其金属 (II) 配合物进行结构表征。研究表明,席夫碱(H 2 L)作为一元三齿ONO配体,根据通式[M(HL) 2符合八面体几何形状。]。此外,利用紫外-可见吸收和粘度测量,在 pH = 7.2 下研究了这些复合物与 CT-DNA 的相互作用。为了确定金属 (II) 配合物与 B-DNA 十二聚体结合的机制,使用 AutoDock Vina 1.2.0 工具进行了对接研究。通过琼脂糖凝胶电泳技术,光诱导切割表明配体 ( H 2 L ) 及其复合物具有针对 pUC19 DNA 的紫外可见光核酸酶特性。研究表明,评估的复合物通过嵌入模式与 CT-DNA 牢固结合,并提供了独特的 DNA 结合模式。

更新日期:2022-05-30
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