Targeted delivery of therapeutic drugs is essential for precise treatment of various diseases to reduce possible serious side-effects. A screened DNA aptamer has been widely developed for active targeting delivery. Herein, we report a facile strategy for the construction of a branched DNA aptamer cluster-based nanoplatform for efficiently targeted drug delivery. In our design, the terminal-modified DNA aptamer can be covalently conjugated to form a branched aptamer cluster by click reaction easily. The branched aptamer cluster-modified DNA tetrahedron (TET) demonstrates highly targeted cellular uptake with the modification of only one site. After loading the chemotherapeutic drug (doxorubicin, DOX), the DNA aptamer cluster-based nanoplatform elicits a remarkable and selective inhibition of tumor cell proliferation by much-enhanced targeted delivery. This covalently conjugated branched DNA aptamer cluster-based nanoplatform provides a new strategy for the development of targeted drug delivery.

中文翻译：

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一种基于共价结合的支链 DNA 适配子簇的纳米平台，用于高效靶向药物递送

治疗药物的靶向递送对于精确治疗各种疾病以减少可能的严重副作用至关重要。已广泛开发筛选的 DNA 适体用于主动靶向递送。在此，我们报告了一种简便的策略，用于构建基于分支 DNA 适配子簇的纳米平台，用于有效靶向药物递送。在我们的设计中，末端修饰的 DNA 适体可以通过点击反应轻松地共价结合形成分支的适体簇。支链适体簇修饰的 DNA 四面体 (TET) 显示出高度靶向的细胞摄取，仅修饰一个位点。加载化疗药物（阿霉素，DOX）后，基于 DNA 适配子簇的纳米平台通过大大增强的靶向递送引起显着和选择性的肿瘤细胞增殖抑制。这种基于共价结合的支链 DNA 适配体簇的纳米平台为靶向药物递送的开发提供了新的策略。