An engineered yeast strain was created by replacing genomic histones with mutants using an optimized yeast genetic code expansion (GCE) system. It can survive with co-translationally modified nucleosomes at the H3K56 position, providing insight into the functional differences in lysine acylation states, which might otherwise be difficult to study. Phenotypic variation between lysine acetylation and crotonylation was observed in this strain.

中文翻译：

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创建具有共翻译酰化核小体的酵母菌株

通过使用优化的酵母遗传密码扩展 (GCE) 系统用突变体替换基因组组蛋白来创建工程酵母菌株。它可以在 H3K56 位置与共翻译修饰的核小体一起存活，从而深入了解赖氨酸酰化状态的功能差异，否则可能难以研究。在该菌株中观察到赖氨酸乙酰化和巴豆酰化之间的表型变异。