The catalytic generation of H₂ in living cells provides a method for antioxidant therapy. In this study, an [FeFe]-hydrogenase mimic [Ru + Fe₂S₂@F127(80)] was synthesized by self-assembling polymeric pluronic F-127, catalytic [Fe₂S₂] sites, and photosensitizer Ru(bpy)₃²⁺. Under blue light irradiation, hydrated protons were photochemically reduced to H₂, which increased the local pH in living cells (HeLa cells). The generated H₂ was subsequently used as an antioxidant to decrease reactive oxygen species (ROS) levels in living cells (HEK 293T, HepG2, MCF-7, and HeLa cells). Our findings revealed that the proliferation of HEK 293T cells increased by a factor of about six times, relative to that of other cells (HepG2, MCF-7, and HeLa cells). Intracellular ROS and pH levels were then monitored using fluorescent cell imaging. Our study showed that cell imaging can be used to evaluate the ability of Ru + Fe₂S₂@F127 to eliminate oxidative stress and prevent ROS-related diseases.

在活细胞中催化产生H ₂为抗氧化治疗提供了一种方法。在这项研究中，_通过自组装聚合物普朗尼克 F-127、催化 [Fe 2 S ₂ ]_位点和光敏剂 Ru( _bpy ) ₃ ²⁺。在蓝光照射下，水合质子被光化学还原为 H ₂，这增加了活细胞（HeLa 细胞）中的局部 pH 值。生成的 H ₂随后被用作抗氧化剂以降低活细胞（HEK 293T、HepG2、MCF-7 和 HeLa 细胞）中的活性氧 (ROS) 水平。我们的研究结果表明，与其他细胞（HepG2、MCF-7 和 HeLa 细胞）相比，HEK 293T 细胞的增殖增加了约 6 倍。然后使用荧光细胞成像监测细胞内 ROS 和 pH 水平。我们的研究表明，细胞成像可用于评估 Ru + Fe ₂ S ₂ @F127 消除氧化应激和预防 ROS 相关疾病的能力。