Zwitterionic sulfobetaine-based monolithic stationary phases have attracted increasing attention for their use in hydrophilic interaction chromatography. In this study, a novel hydrophilic polymeric monolith was fabricated through photo-initiated copolymerization of 3-(3-vinyl-1-imidazolio)-1-propanesulfonate (SBVI) with pentaerythritol triacrylate using methanol and tetrahydrofuran as the porogenic system. Notably, the duration for the preparation of this novel monolith was as little as 5 min, which was significantly shorter than that required for previously reported sulfobetaine-based monoliths prepared via conventional thermally initiated copolymerization. Moreover, these monoliths showed good morphology, permeability, porosity (62.4%), mechanical strength (over 15 MPa), column efficiency (51,230 plates/m), and reproducibility (relative standard deviations for all analytes were lower than 4.6%). Mechanistic studies indicated that strong hydrophilic and negative electrostatic interactions might be responsible for the retention of polar analytes on the zwitterionic SBVI-based monolith. In particular, the resulting monolith exhibited good anti-protein adhesion ability and low nonspecific protein adsorption. These excellent features seem to favor its application in bioanalysis. Therefore, the novel zwitterionic sulfobetaine-based monolith was successfully employed for the highly selective separation of small bioactive compounds and the efficient enrichment of N-glycopeptides from complex samples. In this study, we prepared a novel zwitterionic sulfobetaine-based monolith with good performance and developed a simpler and faster method for preparation of zwitterionic monoliths.

基于两性离子磺基甜菜碱的整体固定相因其在亲水相互作用色谱中的应用而受到越来越多的关注。在这项研究中，使用甲醇和四氢呋喃作为致孔系统，通过 3-(3-vinyl-1-imidazolio)-1-propanesulfonate (SBVI) 与季戊四醇三丙烯酸酯的光引发共聚制备了一种新型亲水性聚合物整体材料。值得注意的是，这种新型整体材料的制备时间仅为 5 分钟，这明显短于先前报道的通过传统热​​引发共聚制备的基于磺基甜菜碱的整体材料所需的时间。此外，这些整体材料表现出良好的形态、渗透性、孔隙率（62.4%）、机械强度（超过 15 MPa）、柱效（51,230 塔板/平方米）、和重现性（所有分析物的相对标准偏差均低于 4.6%）。机理研究表明，强亲水和负静电相互作用可能是极性分析物保留在两性离子 SBVI 基整体上的原因。特别是，所得的整体材料表现出良好的抗蛋白质粘附能力和低的非特异性蛋白质吸附。这些优异的特性似乎有利于其在生物分析中的应用。因此，新型两性离子磺基甜菜碱基整体材料成功用于小生物活性化合物的高选择性分离和高效富集 机理研究表明，强亲水和负静电相互作用可能是极性分析物保留在两性离子 SBVI 基整体上的原因。特别是，所得的整体材料表现出良好的抗蛋白质粘附能力和低的非特异性蛋白质吸附。这些优异的特性似乎有利于其在生物分析中的应用。因此，新型两性离子磺基甜菜碱基整体材料成功用于小生物活性化合物的高选择性分离和高效富集 机理研究表明，强亲水和负静电相互作用可能是极性分析物保留在两性离子 SBVI 基整体上的原因。特别是，所得的整体材料表现出良好的抗蛋白质粘附能力和低的非特异性蛋白质吸附。这些优异的特性似乎有利于其在生物分析中的应用。因此，新型两性离子磺基甜菜碱基整体材料成功用于小生物活性化合物的高选择性分离和高效富集来自复杂样品的N-糖肽。在这项研究中，我们制备了一种新型的具有良好性能的两性离子磺基甜菜碱基整体材料，并开发了一种更简单、更快速的两性离子整体材料的制备方法。