当前位置: X-MOL 学术J. Great Lakes Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Fungal community dynamics associated with harmful cyanobacterial blooms in two Great Lakes
Journal of Great Lakes Research ( IF 2.2 ) Pub Date : 2022-05-26 , DOI: 10.1016/j.jglr.2022.05.007
John A. Marino , Vincent J. Denef , Gregory J. Dick , Melissa B. Duhaime , Timothy Y. James

Harmful algal blooms (HABs) impose major costs on aquatic ecosystems worldwide, including the Laurentian Great Lakes. Microbial consumers, including fungi, can have important interactions with bloom-forming algae and cyanobacteria, although relatively few studies have investigated the relationship between fungi and HABs. We examined changes in the aquatic fungal community coincident with the occurrence of large cyanobacterial blooms in two areas of the Great Lakes (western Lake Erie and Saginaw Bay, Lake Huron). We collected water samples over the course of bloom development, peak, and decline from 3 sites in western Lake Erie on 11 dates and 2 sites in Saginaw Bay on 4 dates. Single molecule sequencing (PacBio RS II) with two molecular markers (the internal transcribed spacer (ITS) of the rRNA locus using fungal-specific primers and the 18S rRNA with primers targeting early-diverging lineages of fungi) was used to estimate fungal community composition. Results indicate a diverse fungal community within the lakes, including several major fungal phyla. The Chytridiomycota were particularly well-represented (54.8% and 45.4% of ITS and 18S sequences, respectively), and we also found representation from both Cryptomycota and Aphelidiomycota, which are putatively obligate intracellular parasites. Further, we found associations between the fungal community (alpha diversity; community composition) and measures of bloom magnitude (chlorophyll a, phycocyanin, and microcystin concentrations) in western Lake Erie. Our results suggest potentially important spatial and temporal heterogeneity in the fungal community that motivates further research on functional importance of fungi in the Great Lakes and consequences for HABs and freshwater ecosystems more broadly.



中文翻译:

两个五大湖中与有害蓝藻水华相关的真菌群落动态

有害藻华(HAB) 给全世界的水生生态系统造成重大损失,包括劳伦森五大湖。微生物消费者,包括真菌,可以与形成水华的藻类和蓝细菌有重要的相互作用,尽管相对较少的研究调查了真菌和 HAB 之间的关系。我们检查了与五​​大湖(西伊利湖和休伦湖的萨吉诺湾)发生大型蓝藻水华同时发生的水生真菌群落变化。我们在 11 个日期从伊利湖西部的 3 个地点和 4 个日期在萨吉诺湾的 2 个地点收集了盛开、高峰和下降过程中的水样。具有两个分子标记的单分子测序 (PacBio RS II)(内部转录使用真菌特异性引物的 rRNA 基因座的间隔区 (ITS) 和具有针对真菌早期发散谱系的引物的 18S rRNA) 用于估计真菌群落组成。结果表明湖泊中有一个多样化的真菌群落,包括几个主要的真菌门。壶菌门的代表性特别好(分别占 ITS 和 18S 序列的 54.8% 和 45.4%),我们还发现了隐菌门和蚜虫门的代表性,它们是假定的专性细胞内寄生虫。此外,我们发现真菌群落(α 多样性;群落组成)与水华强度测量(叶绿素a、藻蓝蛋白和微囊藻毒素浓度)在伊利湖西部。我们的研究结果表明真菌群落中潜在的重要空间和时间异质性,这促使人们进一步研究五大湖真菌的功能重要性以及更广泛地对 HAB 和淡水生态系统的影响。

更新日期:2022-05-26
down
wechat
bug