The present study aimed to evaluate the genotoxic potential of cerium oxide (CeO₂), magnesium oxide (MgO) nanoparticles and their ionic forms by alkaline comet assay. Eisenia hortensis were exposed to different series of concentrations (25, 50, 100, 200, and 400 μg/ml) of chemicals for 48 h to find LC₅₀. The LC₅₀ for MgO and CeO₂ NPs were 70 and 80 μg/ml. Whereas, the LC₅₀ for their ionic forms were 50 and 70 μg/ml. To assess the potential DNA damage caused by the chosen chemicals, E. hortensis was further exposed for 48 h to the following concentrations, based on their respective LC_50s: LC_50/2, LC⁵⁰, and 2xLC₅₀. Comet scores demonstrated the significant increase (p < 0.05) in DNA damage at all concentrations, both for NPs and ionic forms in a concentration-dependent manner. Findings of the present study revealed the genotoxic effects of CeO₂ NPs, MgO NPs and their ionic forms on E. hortensis.

中文翻译：

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用碱性彗星法测定 Eisenia hortensis 体腔细胞中铈和镁纳米粒子及其离子形式的基因毒性

本研究旨在通过碱性彗星试验评估氧化铈 (CeO ₂ )、氧化镁 (MgO) 纳米颗粒及其离子形式的遗传毒性潜力。将Eisenia hortensis暴露于不同系列浓度（25、50、100、200 和 400 μg/ml）的化学品中 48 小时以找到 LC ₅₀。MgO 和 CeO ₂ NPs的 LC ₅₀分别为 70 和 80 μg/ml。然而，它们离子形式的 LC ₅₀分别为 50 和 70 μg/ml。为了评估所选化学物质造成的潜在 DNA 损伤，将E. hortensis进一步暴露于以下浓度 48 小时，基于它们各自的 LC _50s：LC _50/2，LC⁵⁰和 2xLC ₅₀。 Comet 评分表明，在所有浓度下，NPs 和离子形式的 DNA 损伤均以浓度依赖性方式显着增加 ( p < 0.05)。本研究的结果揭示了 CeO ₂ NPs、MgO NPs 及其离子形式对E. hortensis的遗传毒性作用。