Hydrogen sulfide (H₂S) is an essential intracellular gasotransmitter undertaking numerous signaling roles and physiological effects. Herein, an ingenious anisotropic gold (Au)-silver (Ag) Janus plasmonic nanoprobe was constructed based on the dark-field light scattering imaging for the detection of exogenous and endogenous H₂S. In the presence of sulfide, the Ag islands as the sensing agent were susceptible to oxidation to Ag₂S, which induced changes in the longitudinal localized surface plasmon resonance (LSPR) properties of Au-Ag Janus nanoparticles (JNPs), resulting in remarkable and distinctive scattering color changes as well as spectral shifts. The JNPs exhibited satisfying anti-interference capability and a good linear relationship with a low detection limit of 0.11 nM. Additionally, this distinct nanoprobe was successfully applied to monitor endogenous H₂S as well as map local variations of H₂S in real-time with its unique intuition and sensitivity in intracellular imaging applications. This work provided a promising application in the plasmonic detection of intracellular H₂S and paved insight into the H₂S-related biological process.

硫化氢 (H ₂ S) 是一种重要的细胞内气体递质，具有多种信号传导作用和生理作用。在此，基于暗场光散射成像构建了一种巧妙的各向异性金（Au）-银（Ag）Janus等离子体纳米探针，用于检测外源性和内源性H ₂ S。在硫化物存在下，Ag岛作为传感剂易被Ag _2氧化S，它引起 Au-Ag Janus 纳米粒子 (JNPs) 的纵向局部表面等离子体共振 (LSPR) 特性的变化，导致显着和独特的散射颜色变化以及光谱偏移。JNPs表现出令人满意的抗干扰能力和良好的线性关系，检测限低至0.11 nM。此外，这种独特的纳米探针成功地应用于监测内源性 H ₂ S 以及实时绘制 H _{2 S 的局部变化，其在细胞内成像应用中具有独特的直觉和灵敏度。这项工作为细胞内 H 2} S的等离子体检测提供了有前景的应用，并为深入了解与 H ₂ S 相关的生物过程奠定了基础。