Solid-phase microextraction (SPME)–direct mass spectrometry (MS) has proven to be an efficient tool for the rapid screening and quantitation of target compounds at trace levels. However, it is challenging to perform screening using both positive and negative modes in one analytical run without compromising scanning speed and detection sensitivity. To take advantage of the special geometry of a coated blade spray (CBS) blade, which consists of two flat sides coated with the same SPME coating, we developed a CBS-MS method that enables desorption and ionization to be performed in positive ionization mode on one side of a coated blade and negative ionization mode on the other side of the same blade. By simply flipping the blade 180°, MS analysis in both ionization modes on different sides can be completed in 40 s. Combining this approach with an automated Concept 96-blade-based SPME system allowed analysis for one sample in positive and negative modes to be completed in less than 1 min. The workflow was optimized by using a biocompatible polyacrylonitrile as an undercoating layer and a binder of polyacrylonitrile/hydrophilic–lipophilic balance (HLB) particles, which enabled the rapid analysis of 20 drugs of abuse in saliva samples in both positive and negative modes. The proposed method provided low limits of quantification (between 0.005 and 10 ng/mL), with calibration linear correlation coefficients ⩾ 0.9925, accuracy between 72% and 126%, and relative precision < 15% for three validation points.

中文翻译：

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通过涂层刀片喷雾-质谱法通过正负模式快速筛选和定量滥用药物

固相微萃取 (SPME) - 直接质谱 (MS) 已被证明是快速筛选和定量痕量目标化合物的有效工具。然而，在一次分析运行中同时使用正模式和负模式进行筛选而不影响扫描速度和检测灵敏度是一项挑战。为了利用涂层叶片喷雾 (CBS) 叶片的特殊几何形状，它由两个涂有相同 SPME 涂层的平面组成，我们开发了一种 CBS-MS 方法，可以在正离子模式下进行解吸和电离。带涂层刀片的一侧和同一刀片另一侧的负电离模式。只需将刀片翻转 180°，即可在 40 秒内完成不同侧两种电离模式的 MS 分析。将此方法与基于 Concept 96 刀片的自动化 SPME 系统相结合，可以在不到 1 分钟的时间内完成对一个样品的正负模式分析。通过使用生物相容性聚丙烯腈作为底涂层和聚丙烯腈/亲水-亲油平衡 (HLB) 颗粒粘合剂对工作流程进行了优化，从而能够以阳性和阴性模式快速分析唾液样品中的 20 种滥用药物。所提出的方法提供了低定量限（0.005 和 10 ng/mL 之间），校准线性相关系数  0.9925，准确度在 72% 和 126% 之间，三个验证点的相对精度 < 15%。通过使用生物相容性聚丙烯腈作为底涂层和聚丙烯腈/亲水-亲油平衡 (HLB) 颗粒粘合剂对工作流程进行了优化，从而能够以阳性和阴性模式快速分析唾液样品中的 20 种滥用药物。所提出的方法提供了低定量限（0.005 和 10 ng/mL 之间），校准线性相关系数  0.9925，准确度在 72% 和 126% 之间，三个验证点的相对精度 < 15%。通过使用生物相容性聚丙烯腈作为底涂层和聚丙烯腈/亲水-亲油平衡 (HLB) 颗粒粘合剂对工作流程进行了优化，从而能够以阳性和阴性模式快速分析唾液样品中的 20 种滥用药物。所提出的方法提供了低定量限（0.005 和 10 ng/mL 之间），校准线性相关系数  0.9925，准确度在 72% 和 126% 之间，三个验证点的相对精度 < 15%。