Sauropus spatulifolius Beille (S. spatulifolius) is a commonly used medicine of the Bourau and Yao nationalities. However, the composition of S. spatulifolius is complex, and simple chemical fingerprints cannot accurately evaluate the relationship between its composition and efficacy. In this study, high-performance liquid chromatography (HPLC) method was used to establish the fingerprint of the ethyl acetate extract of S. spatulifolius. Based on the evaluation of the similarity of chromatographic fingerprints of traditional Chinese medicine, combined with cluster analysis and principal component analysis (PCA), the common peaks of fingerprints were evaluated. The anti-inflammatory effect data were extracted through the dimethylbenzene-induced ear-swelling model in mice. The gray relational analysis (GRA) combined with partial least squares regression (PLSR) was used to study the spectrum-effect correlation of S. spatulifolius. As a result, the HPLC fingerprint of the ethyl acetate extract of S. spatulifolius was established, and 18 common peaks were identified. Except for S6, the other similarities are all above 0.915. The reference substance control method was used to identify two absorption peaks, namely, protocatechuic acid and caffeic acid. The cluster analysis results showed that 10 samples from different origins were grouped into four categories, which was consistent with the PCA results. Ethyl acetate extract of 10 batches of S. spatulifolius could significantly inhibit the ear swelling of mice (). Through GRA, the order of the contribution of each chemical component to the anti-inflammatory efficacy was obtained. The results of PLSR showed that the VIP values of peaks 3, 4, and 12 were greater than 1 and were positively correlated with anti-inflammatory activity. In this study, the HPLC fingerprint of the ethyl acetate extract of S. spatulifolius was established. Through the study of the spectrum-effect correlation, the anti-inflammatory active substance of the ethyl acetate extract of S. spatulifolius was obtained. The anti-inflammatory effect of S. spatulifolius was the result of the joint action of multiple ingredients. This research helps to quickly and accurately discover the active ingredient groups of traditional Chinese medicine and provides new ideas and methods for studying the effective substances of traditional Chinese medicine.

中文翻译：

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三叶三叶草抗炎活性提取物的谱效相关性研究

三叶三叶草（S. spatulifolius ）是布劳族和瑶族的常用药材。然而，S. spatulifolius的成分复杂，简单的化学指纹无法准确评估其成分与功效之间的关系。本研究采用高效液相色谱 (HPLC) 方法建立七叶草乙酸乙酯提取物的指纹图谱。. 在评价中药色谱指纹图谱相似性的基础上，结合聚类分析和主成分分析（PCA），对指纹图谱的共同峰进行评价。通过二甲苯致小鼠耳肿胀模型提取抗炎作用数据。采用灰色关联分析(GRA)结合偏最小二乘回归(PLSR)方法研究S. spatulifolius的光谱-效应相关性。结果， S. spatulifolius乙酸乙酯提取物的 HPLC 指纹图谱建立，并确定了 18 个共同峰。除S6外，其他相似度均在0.915以上。采用对照品对照法鉴定出原儿茶酸和咖啡酸两个吸收峰。聚类分析结果表明，10个不同来源的样本被分为四类，与PCA结果一致。10批八角草乙酸乙酯提取物对小鼠耳肿胀有显着抑制作用。）。通过GRA，获得了每种化学成分对抗炎功效的贡献顺序。PLSR结果显示，3、4、12峰的VIP值均大于1，与抗炎活性呈正相关。在本研究中，建立了七叶草乙酸乙酯提取物的 HPLC 指纹图谱。通过光谱-效应相关性的研究，得到了八角草乙酸乙酯提取物的抗炎活性物质。S. spatulifolius的抗炎作用是多种成分共同作用的结果。本研究有助于快速、准确地发现中药有效成分群，为研究中药有效物质提供新思路和新方法。